STAPHYLOCOCUS AUREUS STRAIN CYL1892
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Abstract
This disclosure presents embodiments of novel strains of Staphylococcus aureus that through genetic engineering produce type 5 capsular polysaccharide at greater levels than Staphylococcus aureus strain Reynolds.
10 Citations
18 Claims
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1-12. -12. (canceled)
- 13. A strain of Staphylococcus aureus comprising a constitutive promoter wherein said constitutive promoter is operably linked to a cap5 operon, wherein said strain produces type 5 capsular polysaccharide at greater levels than wild-type S. aureus strain Reynolds.
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15. A strain of Staphylococcus aureus comprising a constitutive promoter wherein said constitutive promoter is operably linked to a cap5 operon wherein said constitutive promoter is the cap1 promoter of Staphylococcus aureus strain M comprising SEQ ID NO 2, and wherein the cap1 promoter and cap5 operon comprise the deoxyribonucleic acid sequence SEQ ID NO 6.
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16. An isolated deoxyribonucleic acid sequence comprising SEQ ID NO 7.
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17. A method of producing type 5 capsular polysaccharide comprising:
- (a) culturing a strain of Staphylococcus aureus comprising a constitutive promoter wherein said constitutive promoter is operably linked to a cap5 operon, wherein said strain produces type 5 capsular polysaccharide at greater levels than wild-type S. aureus strain Reynolds; and
(b) obtaining type 5 capsular polysaccharide from the culture.
- (a) culturing a strain of Staphylococcus aureus comprising a constitutive promoter wherein said constitutive promoter is operably linked to a cap5 operon, wherein said strain produces type 5 capsular polysaccharide at greater levels than wild-type S. aureus strain Reynolds; and
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18. A method of producing type 5 capsular polysaccharide comprising:
- (a) culturing a strain of Staphylococcus aureus comprising a constitutive promoter wherein said constitutive promoter is operably linked to a cap5 operon wherein said constitutive promoter is the cap1 promoter of Staphylococcus aureus strain M comprising SEQ ID NO 2; and
(b) obtaining type capsular polysaccharide from the culture.
- (a) culturing a strain of Staphylococcus aureus comprising a constitutive promoter wherein said constitutive promoter is operably linked to a cap5 operon wherein said constitutive promoter is the cap1 promoter of Staphylococcus aureus strain M comprising SEQ ID NO 2; and
Specification