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IN VITRO RECOMBINATION METHOD

  • US 20100184187A1
  • Filed: 03/30/2010
  • Published: 07/22/2010
  • Est. Priority Date: 08/11/2005
  • Status: Active Grant
First Claim
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1. A kit for in vitro joining a plurality of dsDNA molecules, comprising, in separate containers,(a) a mixture of the isolated proteins(i) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing,(ii) a non strand-displacing DNA polymerase, and(iii) a ligase,wherein the ratios of activities of (i), (ii) and (iii) are effective, when in the presence of a non-processive 5′

  • exonuclease, to achieve in vitro joining of dsDNA molecules, and(b) an isolated non-processive 5′

    exonuclease.

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