Recycling System for Manipulation of Intracellular NADH Availability

• US 20100184195A1
• Filed: 02/12/2010
• Published: 07/22/2010
• Est. Priority Date: 11/02/2001
• Status: Abandoned Application

• Current Assignee: Rice University
• Original Assignee: Rice University
• Inventors: Berrios-Rivera, Susana J., Bennett, George N., San, Ka-Yiu
• Application Number:
  US12/705,170
• Publication Number:
  US 20100184195A1
• Time in Patent Office: Days
• US Class Current: 435/252.330
• CPC Class Codes: C12N 9/0036 acting on NADH or NADPH (1.6)
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12P 7/625 Polyesters of hydroxy carboxylic acids

Abstract

The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.

Claims

1-10. -10. (canceled)

11. A cell comprising a recombinant NADH-recycling system, wherein said system comprises a heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase.

12. The cell of claim 11, wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a nucleotide sequence encoding an NAH+-dependent formate dehydrogenase.

13. The cell of claim 11, wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a yeast nucleotide sequence encoding an NAH+-dependent dehydrogenase.

14. The cell of claim 11, wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a yeast nucleotide sequence encoding an NAH+-dependent formate dehydrogenase.

15. The cell of claim 11, wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a nucleotide sequence from Candida boidinii.

16. The cell of claim 11, wherein said heterologous nucleotide sequence encoding an NAH+-dependent dehydrogenase is a nucleotide sequence from Candida boidinii comprising SEQ ID NO:

1.

17. The cell of claim 11, wherein said cell is a bacterium.

18. The cell of claim 11, wherein said cell is E. coli.

19. The cell of claim 11, wherein said cell is a member of the genus Rhodococcus.

20-47. -47. (canceled)