GENE-ARRAY OPERATION CHIP DEVICE

• US 20100184623A1
• Filed: 01/22/2009
• Published: 07/22/2010
• Est. Priority Date: 01/22/2009
• Status: Abandoned Application

• Current Assignee: Fooyin University
• Original Assignee: Fooyin University
• Inventors: LIN, Shiu-Ru, HSIUNG, Suz-Kai, CHANG, Hui-Jen
• Application Number:
  US12/357,700
• Publication Number:
  US 20100184623A1
• Time in Patent Office: Days
• US Class Current: 506/17
• CPC Class Codes: B01F 25/10 Mixing by creating a vortex flow, e.g. by tangential introduction of flow components
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01J 2219/00466 in a slurry
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00531 essentially square
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C40B 60/12 : for screening libraries

Abstract

A gene-array operation chip device includes a reaction chamber, and a plurality of inlet holes connected respectively to agitation channels for creating agitation currents within the reaction chamber. Each agitation channel has two ends respectively connected to one of the inlet holes and to the reaction chamber. The agitation channels are inclined with respect to radial directions about an axis of the reaction chamber. Preferably, a flow channel interconnecting two reaction chambers has a hydrophobic wall surface to limit a fluid from flowing naturally between the chambers. In an embodiment, the reaction chamber is a hybridization chamber provided with a membrane-array.

Claims

1. A gene-array operation chip device, comprising:

at least one reaction chamber;

a plurality of inlet holes adapted to supply reactants into said reaction chamber; and

a plurality of agitation channels for creating agitation currents within said reaction chamber, each of said agitation channels having two ends respectively connected to one of said inlet holes and to said reaction chamber, said agitation channels being inclined with respect to radial directions about an axis of said reaction chamber.

2. The gene-array operation chip device of claim 1, wherein said agitation channels extend from said inlet holes to said reaction chamber substantially along the same rotational direction.

3. The gene-array operation chip device of claim 1, wherein said inlet holes are spaced apart angularly around said axis of said reaction chamber, and said agitation channels are respectively connected to said inlet holes and are connected to said reaction chamber at angularly spaced apart positions.

4. The gene-array operation chip device of claim 3, comprising a plurality of said reaction chambers including at least one treatment chamber and at least one hybridization chamber fluidly communicated with said treatment chamber, each of said treatment chamber and said hybridization chamber being connected to a plurality of said inlet holes through a plurality of said agitation channels.

5. The gene-array operation chip device of claim 4, comprising a plurality of said treatment chambers including a first treatment chamber, a second treatment chamber in direct fluid communication with said first treatment chamber, and a third treatment chamber in direct fluid communication with said second treatment chamber and said hybridization chamber.

6. The gene-array operation chip device of claim 5, wherein each of said first and third treatment chambers and said hybridization chamber has a round chamber wall.

7. The gene-array operation chip device of claim 5, further comprising a first flow channel interconnecting said first and second treatment chambers, a second flow channel interconnecting said second and third treatment chambers, and a third flow channel interconnecting said third treatment chamber and said hybridization chamber, each of said first, second and third flow channels having a channel confining wall provided with a hydrophobic wall surface to limit the reactants from flowing naturally between said first, second, third treatment and hybridization chambers.

8. The gene-array operation chip device of claim 7, further comprising a plurality of suction channels respectively connected to said second and third treatment chambers and said hybridization chamber.

9. The gene-array operation chip device of claim 8, further comprising a membrane-array provided with a plurality of probes thereon and arranged to allow the reactants in said hybridization chamber to react with said probes.

10. A chip device, comprising

at least two reaction chambers;

at least one flow channel interconnecting said two reaction chambers, and having a channel confining wall provided with a hydrophobic wall surface to limit a fluid from flowing naturally between said two reaction chambers; and

at least one suction channel connected to one of said reaction chambers.

11. The chip device of claim 10, further comprising at least one inlet hole connected to the other one of said reaction chambers.

12. A gene-array operation chip device, comprising:

at least one treatment chamber to obtain a target to be tested from a sample;

a hybridization chamber fluidly communicated with said treatment chamber to receive the target from said treatment chamber; and

a membrane-array provided with a plurality of probes to react with the target in said hybridization chamber.

13. The gene-array operation chip device of claim 12, comprising a plurality of said treatment chambers including a cell lysis chamber for lysis of the sample so as to obtain the target, a purification chamber fluidly communicated with said cell lysis chamber for purification of the target from said cell lysis chamber, and an amplification chamber communicated with said purification chamber for amplification of the purified target, said hybridization chamber being connected to said amplification chamber to receive the amplified target and to permit the amplified target to react with said probes.

14. The gene-array operation chip device of claim 12, further comprising a base layer, a chamber layer attached to a top surface of said base layer and defining said treatment chamber and said hybridization chamber, and a cover layer disposed on top of said chamber layer, said membrane-array being fixed to said top surface of said base layer and being located within said hybridization chamber.

15. The gene-array operation chip device of claim 14, wherein said cover layer covers said treatment chamber and said hybridization chamber, and has a transparent window disposed immediately above said hybridization chamber and said membrane-array.