OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION

• US 20100184627A1
• Filed: 09/11/2009
• Published: 07/22/2010
• Est. Priority Date: 01/19/1999
• Status: Active Grant

• Current Assignee: Codexis, Inc.
• Original Assignee: Maxygen Inc.
• Inventors: Patten, Phillip A., Gustafsson, Claes, Minshull, Jeremy, Stemmer, Willem P.C., Bass, Steven H., Crameri, Andreas, Ness, John E., Welch, Mark
• Granted Patent: US 8,058,001 B2
• Time in Patent Office: Days
• US Class Current: 506/23
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00

Abstract

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

Claims

1. A method of recombining homologous nucleic acids, the method comprising:

(i) hybridizing a set of family gene shuffling oligonucleotides; and

,

(ii) elongating the set of family gene shuffling oligonucleotides, thereby providing a population of recombined nucleic acids.

2-104. -104. (canceled)