MICROSCOPIC IMAGE CAPTURING APPARATUS, MICROSCOPIC IMAGE CAPTURING METHOD, AND STORAGE MEDIUM HAVING A MICROSCOPE IMAGE CAPTURING PROGRAM STORED THEREON
First Claim
1. A microscopic image capturing apparatus comprising:
- a microscope which obtains a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample; and
a digital camera which is attached to the microscope and captures the light images obtained by the microscope;
wherein the digital camera comprises;
an A/D converting unit which A/D-converts an image signal of each of the captured light images;
a shading correction processing unit which executes a shading correction process for digital image data converted by the A/D converting unit;
a correction data storing unit which stores shading correction data used when the shading correction process is executed by the shading correction processing unit; and
a controlling unit;
wherein the correction data storing unit stores first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image acquired by using a median filter for the image data of the light image without the sample, which is obtained by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of removing a defective portion; and
wherein the controlling unit sets selected shading correction data in the shading correction processing unit from the correction data storing unit in correspondence with an externally input selection of using one of the first and the second shading correction data, when the shading correction process is executed for a image data of the light image with the sample obtained by capturing the light image with the sample with the image capturing unit.
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Accused Products
Abstract
In a correction data storing unit of a digital camera which captures a microscopic image, image data of a light image without a sample, image data obtained by using a filter for removing a defective portion for the light image without a sample, image data where a region without being corrected (or a region being corrected) is set in the light image without the sample, and shading correction data calculated from image data obtained by setting a mask region where data after being corrected is set to 0 are stored. An operator obtains, captures, records, and stores an observation image satisfactory for great-looking by using arbitrary shading correction data. In this way, a captured image satisfactory for great-looking is obtained by making a suitable shading correction according to a shading characteristic, which is caused by an optical system.
26 Citations
9 Claims
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1. A microscopic image capturing apparatus comprising:
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a microscope which obtains a light image with a sample illuminated by a light source, which exists within a viewing field, and a light image without the sample removed from the viewing field, which is captured in a state of being only illuminated by the light source, with an objective lens arranged as opposed to the sample; and a digital camera which is attached to the microscope and captures the light images obtained by the microscope; wherein the digital camera comprises; an A/D converting unit which A/D-converts an image signal of each of the captured light images; a shading correction processing unit which executes a shading correction process for digital image data converted by the A/D converting unit; a correction data storing unit which stores shading correction data used when the shading correction process is executed by the shading correction processing unit; and a controlling unit; wherein the correction data storing unit stores first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image acquired by using a median filter for the image data of the light image without the sample, which is obtained by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of removing a defective portion; and wherein the controlling unit sets selected shading correction data in the shading correction processing unit from the correction data storing unit in correspondence with an externally input selection of using one of the first and the second shading correction data, when the shading correction process is executed for a image data of the light image with the sample obtained by capturing the light image with the sample with the image capturing unit. - View Dependent Claims (2, 3)
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4. A microscopic image capturing method in a digital camera, which is attached to a microscope, and which captures a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, which generates digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and which executes a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, the method comprising:
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storing, in the correction data storing device, first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the digital camera, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image, which is acquired by using a median filter for the image data of the light image without the sample acquired by capturing the light image without the sample with the digital camera, in correspondence with a selection of removing a defective portion; and reading from the shading correction data storing device the first or the second shading data, which is selected according to an externally input selection of using one of the first and the second shading correction data in the shading correction process, and using the read first or second shading correction data in the shading correction process. - View Dependent Claims (5, 6)
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7. A computer-readable storage medium having a program stored thereon for controlling a computer, which executes a microscopic image capturing method in a digital camera, which is attached to a microscope, and which captures a light image with a sample where the light image of the sample is within a viewing field, and light image without the sample, from which the sample is removed and which is only illuminated by the light source, within the viewing field of an objective lens, the light images being obtained by the microscope with the objective lens arranged as opposed to the sample illuminated by the light source, which generates digital image data by A/D (analog/digital) converting an image signal of each of the captured light images, and which executes a shading correction process by using shading correction data prestored in a correction data storing device for the digital image data of the light image with the sample, to execute a process, the process comprising:
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storing, in the correction data storing device, first shading correction data obtained based on image data of the light image without the sample, which is acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of non-removing a defective portion, and second shading correction data obtained based on an image, which is acquired by using a median filter for the image data of the light image without the sample acquired by capturing the light image without the sample with the image capturing unit, in correspondence with a selection of removing a defective portion; and reading from the shading correction data storing device the first or the second shading data, which is selected according to an externally input selection of using one of the first and the second shading correction data in the shading correction process, and using the read first or second shading correction data in the shading correction process. - View Dependent Claims (8, 9)
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Specification