NUCLEIC ACID HYDROGEL VIA ROLLING CIRCLE AMPLIFICATION
First Claim
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1. A nucleic acid-containing hydrogel comprising entangled single-stranded nucleic acid molecules, wherein at least a portion of the single-stranded entangled nucleic acid molecules are formed via rolling cycle amplification.
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Abstract
Methods and compositions are provided for producing nucleic acid-based compositions. Methods include enzyme catalyzed or nucleic acid polymer conjugation. Compositions include nucleic acid-containing hydrogels which can be elongated via rolling circle amplification. The hydrogels can encapsulate bioactive agents for drug delivery.
69 Citations
53 Claims
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1. A nucleic acid-containing hydrogel comprising entangled single-stranded nucleic acid molecules, wherein at least a portion of the single-stranded entangled nucleic acid molecules are formed via rolling cycle amplification.
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2. A method for forming a nucleic acid-containing hydrogel, comprising entangling single-stranded nucleic acid molecules, wherein at least a portion of the single-stranded nucleic acid molecules are formed by amplifying a circular nucleic acid template.
- 3. A method for forming a hydrogel, comprising entangling one or more of a first single-stranded nucleic acid molecule and a second single-stranded nucleic acid molecule, wherein the first single-stranded nucleic acid molecule is formed by amplifying a circular nucleic acid template.
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17. A nucleic acid-containing hydrogel, comprising:
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a first single-stranded nucleic acid molecule, the first single-stranded nucleic acid molecule formed from the amplification of a circular nucleic acid template using a first primer; and a second single-stranded nucleic acid molecule, the second single-stranded nucleic acid molecule formed from the amplification of the first single-stranded nucleic acid molecule using a second primer. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A nucleic acid-containing hydrogel, comprising:
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a first single-stranded nucleic acid molecule, the first single-stranded nucleic acid molecule formed from the amplification of a circular nucleic acid template using a first primer; and a second nucleic acid molecule. - View Dependent Claims (28, 29, 30)
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31. A method for forming a hydrogel, comprising:
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hybridizing a circular nucleic acid template with a first primer; forming a first nucleic acid molecule from the first primer; hybridizing a second primer with the first single-stranded nucleic acid molecule; and forming a second nucleic acid molecule from the second primer. - View Dependent Claims (32, 33, 34, 35, 36, 37)
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38. A method of encapsulating one or more compounds in a hydrogel, comprising the steps of:
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a. providing an aqueous solution comprising said one or more compounds; b. mixing said aqueous solution with a mixture comprising a plurality of circular nucleic acid templates; and c. amplifying the circular nucleic acid template to form the hydrogel, thereby encapsulating the one or more compounds in the hydrogel. - View Dependent Claims (39, 40, 41, 42, 43, 44)
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45. A method for delivering a compound, comprising:
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providing a composition comprising single-stranded nucleic acid molecules encapsulating the compound, wherein at least a portion of the single-stranded nucleic acid molecules are formed by rolling circle amplification; and administering the composition to a subject. - View Dependent Claims (46, 47, 48, 49)
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50. A method of cell-free synthesis of one or more proteins, comprising:
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a. providing a composition comprising single-stranded nucleic acid molecules, wherein at least a portion of the single-stranded nucleic acid molecules are formed by rolling circle amplification; and b. expressing the one or more proteins from the nucleic acid hydrogel. - View Dependent Claims (51, 52, 53)
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Specification