THREE-DIMENSIONAL POROUS HYBRID SCAFFOLD AND MANUFACTURE THEREOF

• US 20100190254A1
• Filed: 12/05/2007
• Published: 07/29/2010
• Est. Priority Date: 12/05/2006
• Status: Active Application

• Current Assignee: Nanyang Technological University
• Original Assignee: Nanyang Technological University
• Inventors: Gopal, Pamela E-Wei, Ratner, Buddy Dennis, Leong, Meng Fatt, Chian, Kerm Sin
• Application Number:
  US12/517,891
• Publication Number:
  US 20100190254A1
• Time in Patent Office: Days
• US Class Current: 435/396
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3847 Bones
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: C08J 9/00 Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof mechanical aspects of shaping of plastics or substances in a plastic state for the production of porous or cellular articles B29C
• CPC Class Codes: D01D 5/0076 characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid D01D5/0046 takes precedence
• CPC Class Codes: Y10T 156/10 Methods of surface bonding and/or assembly therefor

Abstract

The present invention refers to a three-dimensional porous hybrid scaffold for tissue engineering and methods of its manufacture and use.

Claims

1. A three-dimensional hybrid scaffold for tissue engineering comprising:

a first layer made of a decellularized biological material;

a second porous layer connected to the surface of said first layer, wherein said second layer is a porous bioadhesive; and

a third porous layer connected to the surface of said second layer which is located opposite the surface to which said first layer is connected, wherein said third layer is a three-dimensional porous polymer scaffold.

2. The hybrid scaffold according to claim 1, wherein said third layer is an electrospun three-dimensional porous scaffold.

3. The hybrid scaffold according to claim 2, wherein said electrospun scaffold is obtained by

providing an electrospinning apparatus;

forming crystals from a molecule or group of molecules, which are in vapor phase comprised in the surrounding atmosphere, at the surface of the collector of said electrospinning apparatus, wherein the reaction chamber of said electrospinning apparatus has a temperature which allows formation of crystals at said surface of said collector;

electrospinning a solution comprising at least one polymer dissolved therein around the crystals;

continuing the formation of crystals and the electrospinning simultaneously; and

removing the crystals by sublimation.

4. The hybrid scaffold according to claim 3, wherein said collector of said electrospinning apparatus is composed of said first and second layer and thus said electrospun scaffold is electrospun directly onto the surface of said second layer.

5. The hybrid scaffold according to claim 1, wherein said biological material is selected from the group consisting of small intestine, liver, pancreas, urinary bladder, stomach, bladder, vascular system, bile duct, alimentary canal, respiratory tract, kidney, spleen, heart, heart valve, bone, skin or fragments or parts thereof.

6. The hybrid scaffold according to claim 1, wherein said biological material is esophageal mucosa.

7. The hybrid scaffold according to claim 1, wherein said biological material is derived from an individual which is selected from the group consisting of mammal, reptiles and insects.

8. The hybrid scaffold according to claim 7, wherein said mammal is selected from the group consisting of porcine, bovine, ovine, rabbit, monkey and human.

9. The hybrid scaffold according to claim 1, wherein said bioadhesive is selected from the group consisting of fibrin glue, polyvinylpyrolidone, polyvinylpyrolidone/vinyl acetate copolymers, polyethylene glycol, platelated gel, chitosan or gelatin-resorcin-formaldehyde.

10. The hybrid scaffold according to claim 9, wherein said bioadhesive is fibrin glue.

11. The hybrid scaffold according to claim 1, wherein the polymer for said three-dimensional porous polymer scaffold is a biodegradable and/or biocompatible polymer.

12. The hybrid scaffold according to claim 1, wherein said second porous layer and said third layer have a pore size which is between about 10 nm to about 500 μ

m.

13. The hybrid scaffold according to claim 1, wherein the thickness of said bioadhesive layer is about 10 to 1000 μ

m before said third layer is connected to said bioadhesive second layer.

14. A method of manufacturing a three-dimensional hybrid scaffold according to claim 1, the method comprising:

providing a first layer made of a biological material which has been decellularized;

applying a second layer to the surface of said first layer, wherein said second layer is a bioadhesive and wherein said second layer is applied to said first layer at a temperature of the environment which is at or below the freezing temperature of said bioadhesive; and

applying a third layer which is a three-dimensional porous scaffold to a side of said second layer which is not the side facing said first layer.

15. The method according to claim 14, wherein said third layer is comprised of an electrospun scaffold.

16. The method according to claim 14, wherein said third layer is comprised of an electrospun scaffold which is obtained by the process referred to in claim 3.

17. The method according to claim 14, wherein said bioadhesive layer is applied to said first layer using a method selected from the group consisting of spraying, electrospraying, electrospinning, spin coating, dip coating, casting and brushing.

18. The method according claim 14, wherein said third layer is applied before the bioadhesive is fully set/crosslinked.

19. The method according to claim 14, further comprising controlling the degradation time of said bioadhesive layer by controlling the pore size of said bioadhesive layer which is achieved by increasing or decreasing the temperature in the environment when applying the bioadhesive layer, as long as said temperature is still at or below the freezing point of said bioadhesive.

20. The method according to claim 14, wherein said bioadhesive is selected from the group consisting of fibrin glue, polyvinylpyrolidone, chitosan and gelatin-resorcin-formaldehyde.

21. The method according to claim 20, wherein said bioadhesive is fibrin glue.

22. A medicament comprising a three-dimensional hybrid scaffold according to claim 1 for autologous, allogenic, xenogenic transplantation of tissue.

23. The medicament according to claim 22, wherein said tissue bladder, vascular system, bile duct, alimentary canal, respiratory tract, kidney, spleen, heart, heart valve, bone, skin or fragments or parts thereof.

24. (canceled)