Generation of brachial, thoracic and lumbar spinal motor neurons from embryonic stem cells in the absence of all-trans retinoic acid supplement
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Abstract
Disclosed are methods for generating a neuron expressing Hoxc8 transcription factor or a caudal motor neuron comprising culturing an embryonic stem cell in a composition which is essentially free of retinoids and comprises an isotonic salt solution, so as to generate the neuron which expresses Hoxc8 transcription factor or the caudal motor neuron. Disclosed are also methods for generating a caudal brachial motor neuron, a thoracic motor neuron, or a lumbar motor neuron from an embryonic stem cell in a composition essentially free of retinoids and comprising ADFNK medium, an amount of FGF-2, or Gdf11 respectively. Disclosed are also methods of transplanting a motor neuron into a subject comprising generating the motor neuron and transplanting the motor neuron into the subject. Disclosed is also a population of motor neuron cells enriched for motor neuron cells expressing Foxp1 and expressing a gene associated with Spinal Muscular Atrophy (SMA) or Amyotrophic Lateral Sclerosis (ALS).
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Citations
103 Claims
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1-9. -9. (canceled)
- 10. A method for generating a caudal brachial motor neuron from an embryonic stem cell, comprising culturing the embryonic stem cell in a composition essentially free of retinoids and comprising an amount of Advanced Dulbecco'"'"'s Modified Eagle'"'"'s Medium/F12 and Neurobasal medium that has been supplemented with 10% Knockout-Serum Replacement (ADFNK medium) effective to produce the caudal brachial motor neuron.
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19-22. -22. (canceled)
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23. A method of transplanting a motor neuron into a subject comprising:
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a) generating a motor neuron by culturing an embryonic stem cell in a composition essentially free of retinoids and comprising an isotonic salt solution, so as to generate the motor neuron; and b) transplanting the motor neuron into the subject. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31)
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36-87. -87. (canceled)
- 88. A population of motor neuron cells enriched for motor neuron cells expressing Foxp1 and expressing a gene associated with Spinal Muscular Atrophy (SMA) or Amyotrophic Lateral Sclerosis (ALS).
- 91. A method for generating a caudal motor neuron comprising culturing an embryonic stem cell in a composition which is essentially free of retinoids and comprises an isotonic salt solution, so as to generate the caudal motor neuron.
Specification