POST-IONIZATION OF NEUTRALS FOR ION MOBILITY OTOFMS IDENTIFICATION OF MOLECULES AND ELEMENTS DESORBED FROM SURFACES
First Claim
1. An apparatus comprising:
- an ion source for repetitively or continuously generating ions and neutrals;
a post-ionization device fluidly coupled to said ion source to post-ionize or fragment at least a fraction of said ions and neutrals;
an ion mobility cell capable of receiving the directly desorbed ions and the post-ionized ions;
an ion extractor, fluidly coupled to said ion-mobility device capable of extracting said ions;
a time-of-flight mass spectrometer fluidly coupled to and accepting said ions and fragment ions from said ion extractor,a position sensitive ion detector fluidly coupled to said time-of-flight mass spectrometer to detect said ions and fragment ions;
a timing controller in electronic communication with said ion source and said ion extractor said timing controller tracking and controlling the time of activation of said ion source and controlling the activation of the post-ionization device and activation of said ion extractor according to a predetermined sequence; and
,a data processing unit for analyzing and presenting data said data processing unit in electronic communication with said ion source, said ion extractor, and said position sensitive ion detector.
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Accused Products
Abstract
The present invention relates to a method and apparatus for ionizing a neutral MALDI desorption plume, and in particular, for efficiently measuring the ionized MALDI desorption plume when post-ionization techniques are combined with a medium pressure MALDI-IM-oTOFMS instrument. Additionally, the present disclosure provides a method and apparatus that simultaneously separates tissue-sample MALDI ions by IM-oTOFMS according to their chemical family. After separation, the MALDI ions are directly compared to the ions created by post-ionizing the co-desorbed neutral molecules with a second laser wherein the second laser is delayed by a few hundred microseconds. The present disclosure further provides novel approaches that enhance the analysis of ions, including the use of giant fullerene internal standards to enhance mass accuracy, and ultraviolet (UV) declustering lasers to generate intact peptides and proteins, either of which may be followed by VUV post-ionization which generates identifiable structural fragments.
40 Citations
20 Claims
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1. An apparatus comprising:
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an ion source for repetitively or continuously generating ions and neutrals; a post-ionization device fluidly coupled to said ion source to post-ionize or fragment at least a fraction of said ions and neutrals; an ion mobility cell capable of receiving the directly desorbed ions and the post-ionized ions; an ion extractor, fluidly coupled to said ion-mobility device capable of extracting said ions; a time-of-flight mass spectrometer fluidly coupled to and accepting said ions and fragment ions from said ion extractor, a position sensitive ion detector fluidly coupled to said time-of-flight mass spectrometer to detect said ions and fragment ions; a timing controller in electronic communication with said ion source and said ion extractor said timing controller tracking and controlling the time of activation of said ion source and controlling the activation of the post-ionization device and activation of said ion extractor according to a predetermined sequence; and
,a data processing unit for analyzing and presenting data said data processing unit in electronic communication with said ion source, said ion extractor, and said position sensitive ion detector. - View Dependent Claims (2, 3, 4, 5)
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6. A method for the collection of mass spectrometric data from a sample, comprising the steps of:
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desorbing a chemical species from said sample which produces a desorbed plume comprising a neutral species and an ionized species; post-ionizing the neutral species generated in the desorbing step thereby creating a post-ionized species; separating the post-ionized species in a drift tube by ion mobility; and
,further separating the chemical species in a time-of-flight mass spectrometer. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification