Grating-based sensor combining label-free binding detection and fluorescence amplification and readout system for sensor
First Claim
1. A grating-based biosensor comprising a substrate having a periodic surface grating structure wherein the periodic grating structure is constructed in a manner designed for optical interrogation of the sensor with excitation light at least two discrete wavelengths in an evanescent resonance (ER) detection mode to thereby produce a luminescence response from a sample, placed on the biosensor at least two discrete emission wavelengths.
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Abstract
A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. Various applications for the biosensors are described, including cell-based assays for assessing the effect of drug compounds on cell function. A biosensor embodiment optimized for a luminescent response at two different wavelengths is also described. Such luminescent response could be produced by fluorescence (either native or from an attached fluorophore), phosphorescence, chemi-luminescence, or other luminescence technology. Two different luminescence technologies could be combined on the same biosensor chip.
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Citations
15 Claims
- 1. A grating-based biosensor comprising a substrate having a periodic surface grating structure wherein the periodic grating structure is constructed in a manner designed for optical interrogation of the sensor with excitation light at least two discrete wavelengths in an evanescent resonance (ER) detection mode to thereby produce a luminescence response from a sample, placed on the biosensor at least two discrete emission wavelengths.
- 9. A grating-based biosensor comprising a substrate having a periodic surface grating structure wherein the periodic grating structure is constructed in a manner designed for optical interrogation of the sensor with light in an evanescent resonance (ER) detection mode to produce a luminescence response from a sample placed on the biosensor at two discrete wavelengths, and wherein the luminescence response at the two discrete wavelengths is produced from two different types of luminescence.
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