DETECTING ANALYTES USING BOTH AN OPTICAL AND AN ELECTRICAL MEASUREMENT METHOD

• US 20100203516A1
• Filed: 01/25/2008
• Published: 08/12/2010
• Est. Priority Date: 01/25/2007
• Status: Abandoned Application

• Current Assignee: ITI Scotland Limited (Scottish Government)
• Original Assignee: ITI Scotland Limited (Scottish Government)
• Inventors: Beattie, John, Ghazal, Peter, Bachmann, Till, Campbell, Colin, Mount, Andrew
• Application Number:
  US12/524,562
• Publication Number:
  US 20100203516A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/1025 labels being on the same oligonucleotide
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Provided is a method for detecting an analyte, wherein the analyte is labelled with one or more labels relatable to the analyte, which method comprises: a) performing an optical detection method on the labelled analyte to obtain optical data from the one or more labels; b) performing an electrical detection method on the labelled analyte to obtain electrical data from the one or more labels; and c) determining the identity and/or quantity of the analyte from both the optical and electrical data. Further provided is a method for detecting a plurality of analytes, wherein the each different analyte is labelled with one or more different labels relatable to the analyte, which method comprises: a) performing an optical detection method on a plurality of labelled analytes to obtain optical data from the labels; b) performing an electrochemical detection method on the plurality of labelled analytes to obtain electrical data from the labels; and c) determining the identity and/or quantity of the plurality of analytes from both the optical and electrical data.

Claims

1. A method for detecting one or more analytes, wherein the analyte is labelled with one or more labels relatable to the analyte, which method comprises:

a) performing an optical detection method on the labelled analyte to obtain optical data from the one or more labels;

b) performing an electrical detection method on the labelled analyte to obtain electrical data from the one or more labels; and

c) determining the identity and/or quantity of the analyte from both the optical and electrical data.

2. A method according to claim 1, wherein the one or more labels are suitable for optical and electrical detection, and the one or more labels in step (a) are the same as the one or more labels in step (b).

3. A method according to claim 2, wherein before step (a), the method further comprises the step of labelling the analyte with the one or more labels to form the labelled analyte.

4. A method according to claim 1, wherein the one or more labels in step (a) are suitable for optical detection and the one or more labels in step (b) are suitable for electrical detection and the one or more labels in step (a) are different from the one or more labels in step (b).

5. A method according to claim 4, wherein before step (a), the method further comprises the step of labelling the analyte with the one or more labels in step (a) and the one or more labels in step (b) either simultaneously or separately to form the labelled analyte.

6. The method of claim 1, wherein the one or more analytes comprises a plurality of analytes, wherein each different analyte is labelled with one or more different labels relatable to the analyte, which method comprises:

a) performing an optical detection method on a plurality of labelled analytes to obtain optical data from the labels;

b) performing an electrochemical detection method on the plurality of labelled analytes to obtain electrical data from the labels; and

c) determining the identity and/or quantity of the plurality of analytes from both the optical and electrical data.

7. A method according to claim 6, wherein the one or more labels are suitable for optical and electrical detection and the one or more labels for each analyte in step (a) are the same as the one or more labels for each analyte in step (b).

8. A method according to claim 7, wherein before step (a), the method further comprises the step of labelling the plurality of analytes with the one or more labels to form the plurality of labelled analytes.

9. A method according to claim 6, wherein the one or more labels in step (a) are suitable for optical detection and the one or more labels in step (b) are suitable for electrical detection and the one or more labels for each analyte in step (a) are different from the one or more labels for each analyte in step (b).

10. A method according to claim 9, wherein before step (a), the method further comprises the step of labelling the plurality of analytes with the one or more labels in step (a) and the one or more labels in step (b) either simultaneously or separately to form the plurality of labelled analytes.

11. A method according to claim 1, wherein the labels are selected from nanoparticles, single molecules, chemiluminescent enzymes and fluorophores.

12. A method according to claim 11, wherein the labels are nanoparticles comprising a collection of molecules and/or atoms.

13. A method according to claim 12, wherein the nanoparticles are selected from metals, metal nanoshells, metal binary compounds and quantum dots.

14. A method according to claim 13, wherein the nanoparticles are metal compounds selected from CdSe, ZnS, CdTe, CdS, PbS, PbSe, Hgl, ZnTe, GaAs, HgS, CdAs, CdP, ZnP, AgS, InP, GaP, GaInP, and InGaN.

15. A method according to claim 13, wherein the nanoparticles are selected from gold, silver, copper, cadmium, selenium, palladium and platinum.

19. A method according to claim 6, wherein the one or more labels for each different analyte have different physical properties.

20. A method according to claim 19, wherein the physical properties are selected from one or more of size, shape and surface roughness.

21. A method according to claim 6, wherein the labels for each different analyte have different compositions.

22. A method according to claim 6, wherein the labels for each different analyte are of different types.

23. A method according to claim 1, wherein the optical detection method is selected from optical emission detection, optical absorbance detection, optical scattering detection, spectral shift detection, surface plasmon resonance imaging, and surface-enhanced Raman scattering from adsorbed dyes.

24. A method according to claim 23, wherein the optical detection method is optical emission detection and comprises the steps of irradiating the labelled analytes with light capable of exciting the labels and detecting the frequency and intensity of light emissions from the labels.

25. A method according to claim 24, wherein the light is laser light.

26. A method according to claim 24, wherein the light is selected from infra-red light, visible light and UV light.

27. A method according to claim 26, wherein the light is white light.

28. A method according to claim 1, wherein the electrical detection method is selected from electrical resistive detection and electrochemical detection.

29. A method according to claim 28, wherein the electrical detection method is electrochemical detection and comprises the steps of

(i) placing the labelled analytes into a solution comprising two electrodes whereby the one or more labels dissolve in the solution;

(ii) applying a deposition potential to the electrodes whereby the one or more labels deposit onto one of the electrodes; and

(iii) detecting the electrochemical signals from the electrode.

30. A method according to claim 29, wherein the deposition potential is −

0.1 V to −

1.0 V.

31. A method according to claim 30, wherein the potential is an AC voltage superimposed on a DC voltage.

32. A method according to claim 31, wherein the AC voltage is about 10 mV superimposed on a DC voltage of about 0.24 V.

33. A method according to claim 29, wherein step (ii) further comprises a step of applying a second potential to the electrodes to generate a redox reaction of the deposited labels.

34. A method according to claim 33, wherein the second potential is from +1.0 to +2.0 V.

35. A method according to claim 33, wherein the redox reaction is oxidation of the deposited labels.

36. A method according to claim 1, wherein the analyte comprises one or more compounds selected from a cell, a protein, a polypeptide, a peptide, a peptide fragment, an amino acid, DNA and RNA.

37. A method according to claim 36, wherein the analyte is DNA or RNA and the step of labelling the analyte or plurality of analytes with the one or more labels in step (a) and the one or more labels in step (b) comprises the following steps:

i. binding a primer to the DNA or RNA, wherein the primer is labelled with one or more labels suitable for electrical detection in step (b); and

ii. extending the primer enzymatically with nucleosides, wherein one or more the nucleosides is labelled with one or more labels suitable for optical detection in step (a).

16-18. -18. (canceled)