Host Cells Used For Production of Recombinant Protein

• US 20100203584A1
• Filed: 09/20/2006
• Published: 08/12/2010
• Est. Priority Date: 09/20/2005
• Status: Abandoned Application

• Current Assignee: Taisho Pharmaceutical Company Limited (Taisho Pharmaceutical Holdings Company Limited)
• Original Assignee: Taisho Pharmaceutical Company Limited (Taisho Pharmaceutical Holdings Company Limited)
• Inventors: Miyazaki, Masao, Taira, Hideharu, Yamashita, Tetsuro
• Application Number:
  US12/067,404
• Publication Number:
  US 20100203584A1
• Time in Patent Office: Days
• US Class Current: 435/69.100
• CPC Class Codes: C12N 2500/92 Medium free of human- or animal-derived components
• CPC Class Codes: C12N 2510/02 Cells for production
• CPC Class Codes: C12N 5/0686 Kidney cells

Abstract

It is an object of the present invention to provide host cells, which are able to proliferate in a serum free medium, which are able to synthesize and secrete a large amount of protein of interest, and which are able to produce a glycoprotein having a sugar chain structure. The present invention provides an established cell line, which is established from distal tubular epithelial cells derived from a mammal.

Claims

1. An established cell line, which is established from distal tubular epithelial cells derived from a mammal.

2. The established cell line according to claim 1, which is used as a host cell for production of a recombinant protein.

3. The established cell line according to claim 1, which is derived from a cat.

4. The established cell line according to claim 1, which has a gene encoding a foreign immortality-imparting factor.

5. The established cell line according to claim 1, which has a foreign selective marker gene.

6. The established cell line according to claim 1, which is able to proliferate in a serum free medium.

7. The established cell line according to claim 1, which is a cell having an accession No. FERM BP-10643.

8. A transformant, which is obtained by introducing a gene encoding a protein of interest to be expressed into the established cell line of claim 1.

9. A method for producing a recombinant protein using the established cell line of claim 1.

10. The method according to claim 9, which comprises:

introducing a foreign gene into a cell line established from distal tubular epithelial cells derived from a mammal to produce transformed cells;

culturing the obtained transformed cells, so as to allow the cells to produce a recombinant protein encoded by the foreign gene; and

recovering the produced recombinant protein.

11. The method according to claim 9, wherein the produced recombinant protein is modified with a sugar chain.

12. A recombinant protein, which is produced by the method of claim 9.