High-speed pcr using high-speed dna polymerase

• US 20100203594A1
• Filed: 05/12/2005
• Published: 08/12/2010
• Est. Priority Date: 05/18/2004
• Status: Abandoned Application

• Current Assignee: Toyoda Gosei Company Limited (Toyota Motor Corporation)
• Original Assignee: Toyoda Gosei Company Limited (Toyota Motor Corporation)
• Inventors: Nakajima, Takashi, Segawa, Masaya, Oka, Masanori
• Application Number:
  US11/596,929
• Publication Number:
  US 20100203594A1
• Time in Patent Office: Days
• US Class Current: 435/91.200
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2527/113 Time

Abstract

[Problems] To achieve a high-speed PCR which can be completed in a period of several minutes and thus can be used for a new application.

[Means for Solving Problems] A method for practicing a high-speed PCR under a temperature change of 10° C./sec or more, characterized in that use is made of a heat-resistant DNA polymerase exhibiting a speed of synthesizing a deoxyribonucleic acid of 100 base/sec or higher, and a reagent kit for a high-speed PCR, or use in the method.

Claims

1. A method for performing a high-speed PCR under a temperature change of 10°

C./sec or greater, the method comprising using a heat-resistant DNA polymerase having a deoxyribonucleic acid synthesis rate of 100 bases/sec or greater.

2. The method as set forth in claim 1, wherein the heat-resistant DNA polymerase originates in Thermococcus.

3. The method as set forth in claim 1, wherein the heat-resistant DNA polymerase originates in Thermococcus kodakaraensis.

4. A composition for high-speed PCR that is performed under a temperature change of 10°

C./sec or greater, the composition comprising a heat-resistant DNA polymerase having a deoxyribonucleic acid synthesis rate of 100 bases/sec or greater.

5. A reagent kit for high-speed PCR that is performed under a temperature change of 10°

C./sec or greater, the reagent kit comprising a heat-resistant DNA polymerase having a deoxyribonucleic acid synthesis rate of 100 bases/sec or greater.