Methods and compounds for phototransfer
First Claim
Patent Images
1. A method for transferring a compound from a first surface to a second surface, comprising:
- a) providing i) a compound attached to a first surface through a photocleavable linker;
ii) a source of electromagnetic radiation; and
iii) a second surface;
b) contacting said second surface with said compound; and
c) illuminating said compound with radiation from said radiation source under conditions such that compound is photocleaved from said first surface and transferred to said second surface.
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Abstract
Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
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Citations
49 Claims
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1. A method for transferring a compound from a first surface to a second surface, comprising:
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a) providing i) a compound attached to a first surface through a photocleavable linker;
ii) a source of electromagnetic radiation; and
iii) a second surface;b) contacting said second surface with said compound; and c) illuminating said compound with radiation from said radiation source under conditions such that compound is photocleaved from said first surface and transferred to said second surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for transferring substances from a bead to a surface, comprising:
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a) providing i) a compound attached to a bead through a photocleavable linker;
ii) a source of electromagnetic radiation; and
iii) a surface;b) bringing said bead into contact with said surface; and c) illuminating said bead with radiation from said radiation source under conditions such that compound is photocleaved from said bead and transferred to said surface. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for transferring substances from a bead to a surface, comprising:
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a) providing i) a compound attached to a bead through a photocleavable linker, said bead further comprising a coding agent attached to said bead through a photocleavable linker;
ii) a source of electromagnetic radiation; and
iii) a surface;b) bringing said bead into contact with said surface; and d) illuminating said bead with radiation from said radiation source under conditions such that said compound and said coding agent are photocleaved from said bead and transferred to said surface. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. A method for transferring a compound from a bead to a surface, comprising:
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a) providing i) a photocleavable biotin-labeled compound attached to an avidin-coated bead;
ii) a source of electromagnetic radiation; and
iii) a surface;b) bringing said bead into contact with said surface; and e) illuminating said bead with radiation from said radiation source under conditions such that compound is photocleaved from said bead and transferred to said surface. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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Specification