CALIBRATION METHODS FOR MULTIPLEXED SENSOR ARRAYS

• US 20100204062A1
• Filed: 11/06/2009
• Published: 08/12/2010
• Est. Priority Date: 11/07/2008
• Status: Abandoned Application

• Current Assignee: University of Southern California
• Original Assignee: University of Southern California
• Inventors: Thompson, Mark, Cote, Richard, Roberts, Richard, Zhou, Chongwu
• Application Number:
  US12/614,239
• Publication Number:
  US 20100204062A1
• Time in Patent Office: Days
• US Class Current: 506/16
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 35/00 Methods or apparatus for measurement or analysis of nanostructures
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 35/00693 Calibration

Abstract

The present invention relates to the calibration of devices using a secondary binding agent or reference material. In one embodiment, the present invention provides a method of calibrating a nanosensor by providing a nanosensor comprising an analyte binder attached to a reference binder, extracting a calibration curve from binding a reference material to the reference binder, and calibrating the nanosensor by using the calibration curve to correct for device variation.

Claims

1. A method of calibrating an analyte response of a nanosensor, comprising:

providing a nanosensor comprising a quantity of an analyte binder and a quantity of a reference binder in a fixed ratio to one another;

calibrating the analyte response of the nanosensor by binding a reference material to a portion of the quantity of the reference binder.

2. The method of claim 1, wherein the analyte binder comprises a biomolecule, polynucleotide, polypeptide, aptamer and/or antibody.

3. The method of claim 1, wherein the nanosensor comprises a nanowire based field effect transistor (FET).

4. The method of claim 1, wherein the reference binder comprises a biomolecule, polynucleotide, polypeptide, aptamer and/or antibody.

5. The method of claim 1, wherein the binding of the reference material to the portion of the quantity of the reference binder is at a high affinity and/or is highly selective.

6. The method of claim 1, wherein the reference binder comprises biotin.

7. The method of claim 1, wherein the reference material comprises avidin.

8. A nanosensor array, comprising:

a nanosensor; and

one or more analyte binders and a plurality of reference binders operatively linked to the nanosensor.

9. The nanosensor array of claim 8, wherein the one or more analyte binders and the plurality of reference binders are in a fixed ratio.

10. The nanosensor array of claim 8, wherein the plurality of reference binders have high affinity for a reference compound.

11. The nanosensor array of claim 8, wherein the nanosensor comprises a nanowire based field effect transistor (FET).

12. The nanosensor array of claim 8, wherein the nanosensor comprises a biosensor.

13. The nanosensor array of claim 8, wherein the one or more analyte binders have an affinity to a biomolecule, polynucleotide, polypeptide, aptamer and/or antibody.

14. A method of calibrating a nanosensor, comprising:

providing a nanosensor comprising an analyte binder attached to a reference binder;

extracting a calibration curve through a process that comprises binding a reference material to the reference binder; and

calibrating the nanosensor by using the calibration curve to correct for device variation.

15. The method of claim 14, wherein the analyte binder comprises a biomolecule, polynucleotide, polypeptide, aptamer and/or antibody.

16. The method of claim 14, wherein the nanosensor comprises a nanowire based field effect transistor (FET).

17. The method of claim 14, wherein the reference binder comprises a biomolecule, polynucleotide, polypeptide, aptamer and/or antibody.

18. The method of claim 14, wherein the binding of the reference material to the reference binder is at a high affinity and/or is highly selective.

19. The method of claim 14, wherein the reference binder comprises biotin.

20. The method of claim 14, wherein the reference material comprises avidin.