MICROARRAY USING LAMINAR FLOW AND METHOD OF PREPARING THE SAME
First Claim
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1. A method of preparing a microarray using an apparatus comprising a plurality of channels and an integration channel connected to the plurality of channels, the method comprising:
- introducing a mixture of a photopolymerizable compound-containing solution and probes into the integration channel via the plurality of channels such that the probes from the channels have laminar flow;
photopolymerizing the solution by irradiating radiation onto the integration channel to produce hydrogel; and
separating the hydrogel from the channel.
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Abstract
A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby obtaining high sensitivity. Since gel can be cut to obtain many pieces, many microarrays can be prepared at once.
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11 Claims
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1. A method of preparing a microarray using an apparatus comprising a plurality of channels and an integration channel connected to the plurality of channels, the method comprising:
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introducing a mixture of a photopolymerizable compound-containing solution and probes into the integration channel via the plurality of channels such that the probes from the channels have laminar flow; photopolymerizing the solution by irradiating radiation onto the integration channel to produce hydrogel; and separating the hydrogel from the channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification