Rapid Analyte Measurement Assay
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Abstract
The present the invention provides methods, devices and systems for rapidly measuring analytes within a biological sample.
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Citations
39 Claims
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1-21. -21. (canceled)
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22. A test strip comprising:
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a working electrode; a counter electrode; and a redox reagent system, the redox reagent system comprising a first redox couple and at least one secondary redox couple, wherein the first redox couple is selected and configured to mediate a target analyte in a sample and provide a primary peak and the at least one secondary redox couple selected and configured so as to provide a secondary peak. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30)
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31. A method of measuring concentration of a target analyte in a sample, the method comprising:
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contacting a sample of no more than 1 μ
L comprising the target analyte with a test strip comprising a working electrode;
a counter electrode; and
a redox reagent system, the redox reagent system comprising a first redox couple and at least one secondary redox couple, wherein the first redox couple is selected and configured to mediate a target analyte in a sample and provide a primary peak and the at least one secondary redox couple selected and configured so as to provide a secondary peak; anddetermining the concentration of the target analyte from the no more than 1 μ
L of sample. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38)
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39. A method of measuring the concentration of glucose in a sample, the method comprising:
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contacting a sample of no more than 1 μ
L comprising glucose with a test strip comprising a working electrode;
a counter electrode; and
a redox reagent system, the redox reagent system comprising a first redox couple and at least one secondary redox couple, wherein the first redox couple is selected and configured to mediate glucose in the sample and provide a primary peak and the at least one secondary redox couple selected and configured so as to provide a secondary peak;applying a square wave excitation voltage to the test strip; measuring a response current, wherein the response current comprises a primary peak from mediation of glucose by the first redox couple and one or more secondary peaks from the at least one secondary redox couple; and determining the concentration of glucose from the no more than 1 μ
L of sample within less than 1 second.
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Specification