METHOD OF ANALYSIS OF A MIXTURE OF BIOLOGICAL AND/OR CHEMICAL COMPONENTS WITH THE USE MAGNETIC PARTICLES AND APPARATUS FOR ITS EMBODIMENT

• US 20100209299A1
• Filed: 03/11/2010
• Published: 08/19/2010
• Est. Priority Date: 03/09/2000
• Status: Active Grant

• Current Assignee: Ovvi Diagnostics Se
• Original Assignee: Magnasense Technologies Oy (LifeAssays AB), Petr Ivanovich Nikitin
• Inventors: VETOSHKO, Petr Mikhailovich, NIKITIN, Petr Ivanovich
• Granted Patent: US 8,865,068 B2
• Time in Patent Office: Days
• US Class Current: 422/69
• CPC Class Codes: G01N 27/745 for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/54333 Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01R 33/1269 of molecules labeled with magnetic beads magnetic particles for bio assay G01N33/54326

Abstract

Disclosed is an apparatus for analysis of a mixture of biological and/or chemical components that exposes a spatially arranged chosen component attached to magnetic particles to a magnetic field and records a magnetic induction signal from which the content of the biological and/or chemical components is determined. The magnetic field is alternating and has a pre-set spectrum at least at two frequencies. The apparatus records the signal at a frequency which is a linear combination of the at least two frequencies while the magnetic particles are exposed to the magnetic field.

Claims

1-14. -14. (canceled)

15. An apparatus for analysis of a mixture of biological and/or chemical components, having:

a chosen component of the mixture being analysed, said component being spatially arranged in a prescribed manner;

magnetic particles attached to the chosen component of the mixture being analysed, directly or through an intermediate material;

a magnetic field generator, within which action said magnetic particles are situated;

a meter of the magnetic induction produced by said magnetic particles;

an output signal receiver;

a block generating the result, which input is connected to the output of the output signal receiver,

wherein the chosen component of the mixture being analysed with magnetic particles attached to this component is grouped in a probe volume;

the magnetic field generator is made so as to allow presetting the frequency spectrum of the magnetic field with spectral components, at least, at two frequencies;

a radio-frequency filter is included, which input is connected to the output of the magnetic induction meter, and the output of the filter is connected to the output of the signal receiver, the filter being tuned to pass the signal at a picked-out frequency, which is a linear combination of the frequencies of said spectral components.

16. An apparatus as defined in claim 15, wherein said linear combination of the frequencies of said spectral components is the sum or the difference of these frequencies.

17. An apparatus as defined in claim 15, wherein the magnetic field generator comprises an alternating current (ac) generator made so as to allow pre-setting the frequency spectrum of its output signal with spectral components, at least, at two frequencies, and an inductive block connected to the output of said ac generator, the output of the inductive block being the output of the magnetic field generator.

18. An apparatus as defined in claim 17, wherein said inductive block serves as said magnetic induction meter.

19. An apparatus as defined in claim 18, wherein said inductive block is made as an inductance coil with no core, the first lead of the coil being connected to the output of said ac generator and the second lead being connected to chassis.

20. An apparatus as defined in claim 18, wherein said inductive block comprises two inductance coils without cores, the first leads of said coils being connected, respectively, to the first and the second output of said ac generator, the second leads of the coils being connected to chassis, and, besides, the first lead of one of said coils being connected to the input of said radio-frequency filter.

21. An apparatus as defined in claim 20, wherein the axes of said coils are tilted with respect to each other.

22. An apparatus as defined in claim 21, wherein the angle between the axes of said coils is 90°

.

23. An apparatus as defined claim 21, wherein a phase regulator is inserted between one of the outputs of said ac generator and the associated first lead of one of said coils, said phase regulator being provided with a control input for entering the data on the angle that the axis of this coil makes with the axis of the other coil.

24. An apparatus as defined in claim 15, wherein the magnetic induction meter comprises an inductive element with no core, said element being not a part of said magnetic field generator.

25. An apparatus as defined in claim 15, wherein the magnetic induction meter comprises a magnetoresistive (magneto-impedance) sensitive element.

26. An apparatus as defined in claim 15, wherein the magnetic induction meter comprises a sensitive element based on the Hall effect.

27. An apparatus as defined in claim 15, wherein the magnetic induction meter is made as a planar microelectronic structure.

28. An apparatus as defined in claim 15, wherein the radio-frequency filter has the property to reject the signal of that of the frequencies of said spectral components, which is the nearest to said picked-out frequency.

29. An apparatus as defined in claim 17, wherein the radio-frequency filter has the property to reject the signal of that of the frequencies of said spectral components, which is the nearest to said picked-out frequency, and, besides, the radiofrequency filter is controllable, the control input of the radio-frequency filter being connected to the control output of said ac generator.

30. An apparatus as defined in claim 17, wherein a reference signal generator is inserted, which is connected through its input to the output of said ac generator, through the second, control, input to the output of the output signal receiver, and through its output to the second input of said radio-frequency filter, said filter being made lock-in.

31. An apparatus as defined in claim 17, wherein a control block is inserted, which first and second outputs are connected to the control inputs of said ac generator and said radio-frequency filter, respectively, and the input is connected to the control output of the output signal receiver.

32. An apparatus as defined in claim 30, wherein the reference signal generator and the output signal receiver are made as a processor.

33. An apparatus as defined in claim 30, wherein the output signal generator, the output signal receiver, and said ac generator are made as a processor.

34. An apparatus as defined in claim 15, wherein the magnetic particles are made of a soft magnetic material.

35. An apparatus as defined in claim 15, wherein in said probe volume a working surface is formed, to which the chosen component of the mixture being analysed is bound, said magnetic particles being attached to this component.

36. An apparatus as defined claim 35, wherein the working surface is formed in the probe volume filled with micro-granules.

37. An apparatus as defined in claim 36, wherein said micro-granules are made of polyethylene.

38. An apparatus as defined in claim 37, wherein said micro-granules are produced under low pressure from polyethylene stabilised with gamma-radiation.

39. An apparatus as defined in claim 35, wherein the working surface is formed in the probe volume filled with a capillary-porous structure.

40. An apparatus as defined in claim 35, wherein on the working surface a reagent is immobilised, which is capable of selectively binding the analyte, the chosen component of the mixture being analysed being bound to said reagent, and, through this reagent, to the working surface.

41. An apparatus as defined in claim 15, wherein said probe volume consists of several spatially separated regions, each of these regions being provided with a separate magnetic induction meter, which output is connected to a radio-frequency filter and an output signal receiver.

42. An apparatus as defined in claim 15, wherein said probe volume consists of several spatially separated regions and is made so as to enable successive testing of said regions with a magnetic induction meter.

43. An apparatus as defined in claim 18, wherein the ac generator and the output signal receiver are made as a processor.

44. An apparatus as defined in claim 24, wherein the magnetic induction meter is made as a planar microelectronic structure.