IMMOBILIZED ENZYMES AND USES THEREOF

• US 20100209968A1
• Filed: 05/05/2008
• Published: 08/19/2010
• Est. Priority Date: 05/04/2007
• Status: Abandoned Application

• Current Assignee: Akermin, Inc.
• Original Assignee: Akermin, Inc.
• Inventors: Kesmez, Mehmet, McLaren, James Stirling, Akers, Niki L., Minteer, Shelley D., Zvosec, Richard T., Gellett, Wayne
• Application Number:
  US12/598,888
• Publication Number:
  US 20100209968A1
• Time in Patent Office: Days
• US Class Current: 435/68.100
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/089 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/10 the carrier being a carbohydrate
• CPC Class Codes: H01M 4/90 Selection of catalytic material
• CPC Class Codes: Y02E 60/50 Fuel cells

Abstract

The present invention generally relates to uses of immobilized enzymes. Immobilized enzymes can be used for various chemical transformations, separations, and purifications and can be used in sensors and diagnostics

Claims

1-57. -57. (canceled)

58. An enzyme immobilized in a micellar or inverted micellar immobilization material capable of immobilizing and stabilizing the enzyme, the material being permeable to a compound smaller than the enzyme and either:

(a) when exposed to a temperature of at least about 65°

C. for at least about 18 hours, the stabilized enzyme retains at least about 12% of an activity of an otherwise identical free enzyme exposed to room temperature for the same amount of time;

(b) when exposed to a temperature of at least about 95°

C. for at least about 18 hours, the stabilized enzyme retains at least about 1% of an activity of an otherwise identical free enzyme exposed to room temperature for the same amount of time;

(c) when exposed to a pH of greater than about 12.5 for at least about 18 hours, the stabilized enzyme retains at least about 9% of an activity of an otherwise identical free enzyme exposed to its optimal pH for the same amount of time;

or

(d) when exposed to a pH of less than about 2, the stabilized enzyme retains at least about 10% of its initial bioelectrocatalytic activity for at least about 1 hour when continuously catalyzing a chemical transformation.

59. The immobilized enzyme of claim 58 wherein when exposed to a temperature of at least about 65°

C. for at least about 18 hours, the stabilized enzyme retains at least about 12% of an activity of an otherwise identical free enzyme exposed to room temperature for the same amount of time.

60. The immobilized enzyme of claim 58 wherein when exposed to a temperature of at least about 95°

C. for at least about 18 hours, the stabilized enzyme retains at least about 1% of an activity of an otherwise identical free enzyme exposed to room temperature for the same amount of time.

61. The immobilized enzyme of claim 58 wherein when exposed to a pH of greater than about 12.5 for at least about 18 hours, the stabilized enzyme retains at least about 9% of an activity of an otherwise identical free enzyme exposed to its optimal pH for the same amount of time.

62. The immobilized enzyme of claim 58 wherein when exposed to a pH of less than about 2, the stabilized enzyme retains at least about 10% of its initial bioelectrocatalytic activity for at least about 1 hour when continuously catalyzing a chemical transformation.

63. The immobilized enzyme of claim 59 wherein the stabilized enzyme retains at least about 25% of the activity of an otherwise identical free enzyme exposed to room temperature for the same amount of time.

64. The immobilized enzyme of claim 60 wherein the stabilized enzyme retains at least about 5% of the activity of an otherwise identical free enzyme exposed to room temperature for the same amount of time.

65. The immobilized enzyme of claim 61 wherein the stabilized enzyme retains at least about 25% of an activity of an otherwise identical free enzyme exposed to its optimal pH for the same amount of time.

66. The immobilized enzyme of claim 62 wherein the stabilized enzyme retains at least about 30% of its initial bioelectrocatalytic activity for at least about 48 hours.

67. The immobilized enzyme of claim 58 wherein the enzyme comprises a lipase, a glucose isomerase, a nitrilase, a glucose oxidase, a protease, a carbonic anhydrase, a pepsin, an amylase, a fungal amylase, a maltogenic amylase, a cellulase, a lactase, an esterase, a carbohydrase, a hemicellulase, a pentosanase, a xylanase, a pullulanase, a β

-glucanase, an acetolactate decarboxylase, a β

-glucosidase, a glutaminase, a penicillin acylase, a chloroperoxidase, an aspartic β

-decarboxylase, a cyclodextrin glycosyltransferase, a subtilisin, an aminoacylase, an alcohol dehydrogenase, an amino acid oxidase, a phospholipase, a urease, a cholesterase, a desulfinase, a lignin peroxidase, a pectinase, an oxidoreductase, a dextranase, a glucosidase, a galactosidase, a glucoamylase, a maltase, a sucrase, an invertase, a naringanase, a bromelain, a ficin, a papain, a pepsin, a peptidase, a chymosin, a thermolysin, a trypsin, a triglyceridase, a pregastric esterase, a phosphatase, a phytase, an amidase, a glutaminase, a lysozyme, a catalase, a dehydrogenase, a peroxidase, a lyase, a fumarase, a histadase, an aminotransferase, a ligase, a cyclase, a racemase, a mutase, an oxidase, a reductase, a ligninase, a laccase, a chloroperoxidase, a haloperoxidase, a hydrogenase, a nitrogenase, an oxynitrilase, or a combination thereof.

68. The immobilized enzyme of claim 58 wherein the enzyme comprises a lipase.

69. The immobilized enzyme of claim 58 wherein the immobilization material comprises a cation modified perfluoro sulfonic acid-PTFE copolymer or cation-modified alginate modified with a hydrophobic cation larger than NH₄⁺.

70. The immobilized enzyme of claim 69 wherein the hydrophobic cation comprises tetraethylammonium, tetrapropylammonium (T3A), tetrapentylammonium (T5A), tetrahexylammonium (T6A), tetraheptylammonium (T7A), trimethylicosylammonium (TMICA), trimethyloctyldecylammonium (TMODA), trimethylhexyldecylammonium (TMHDA), trimethyltetradecylammonium (TMTDA), trimethyloctylammonium (TMOA), trimethyldodecylammonium (TMDDA), trimethyldecylammonium (TMDA), trimethylhexylammonium (TMHA), tetrabutylammonium (TBA), triethylhexylammonium (TEHA), and combinations thereof.

71. A particle comprising a core coated with an immobilized enzyme of claim 58.

72. The particle of claim 71 wherein the enzyme is entrapped within the immobilization material.

73. A process for catalyzing a reaction comprising catalyzing the reaction with an immobilized enzyme wherein the enzyme is immobilized in an enzyme immobilization material that immobilizes and stabilizes the enzyme, the material being permeable to a compound smaller than the enzyme, and wherein the reaction catalyzed is selected from

(a) conversion of carbon dioxide to carbonic acid;

(b) enantioselective oxidation or reduction of organic substrates with optional cofactor regeneration;

(c) reactive separation of a component or components from a mixture;

(d) esterification of a carboxylic acid with an alcohol;

(e) liquefaction of corn or other cereals;

(f) saccharification of corn or other cereals to convert starch into sugars;

(g) isomerization of glucose to fructose;

(h) synthesis of chiral compounds;

(i) interesterification of oils;

(j) degumming oil;

(k) treating wastewater (reaction);

(l) clarifying fruit juice;

(m) producing glucose by the starch process;

(n) producing glucose and galactose from lactose;

(o) synthesizing compounds having peptide bonds;

(p) producing 6-aminopenicillic acid from penicillin G;

(q) converting sugars to alcohol;

(r) removing sulfur from petroleum fractions;

(s) converting acrylonitrile to acrylamide;

(t) converting 3-cyanopyridine to nicotinamide; and

(u) degrading stains in a laundry soil.

74. The process of claim 73 wherein the reactive separation of a component or components from a mixture comprises separating or removing the substance from the mixture using an immobilized enzyme wherein the enzyme is entrapped in a micellar or inverted micellar enzyme immobilization material.

75. The process of claim 74 wherein the mixture is a racemic mixture, petroleum product, wastewater, biomass, or food.

76. The process of claim 73 wherein the reaction catalyzed is conversion of carbon dioxide to carbonic acid.

77. The process of claim 73 wherein the enzyme is entrapped in a micellar or inverted micellar immobilization material.