SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS
First Claim
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1. A method of making a fuel from biomass material, the method comprising:
- a. providing a biomass material comprising a high molecular weight carbohydrate;
h. hydrolyzing the biomass material to provide a hydrolyzed biomass material;
c. combining the hydrolyzed biomass material with Clostridium phytofermentans cells in a medium, wherein a concentration of carbohydrates in the medium is greater than 20 mM; and
d. fermenting the hydrolyzed biomass material under conditions and for a time sufficient to produce a fuel.
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Abstract
Clostridium phytofermentans cells (American Type Culture Collection 700394T) and all other strains of the species can ferment materials such as biomass into useful products and coproducts, such as ethanol, hydrogen and organic acids. Compositions that include Clostridium phytofermentans are also disclosed.
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Citations
45 Claims
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1. A method of making a fuel from biomass material, the method comprising:
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a. providing a biomass material comprising a high molecular weight carbohydrate; h. hydrolyzing the biomass material to provide a hydrolyzed biomass material; c. combining the hydrolyzed biomass material with Clostridium phytofermentans cells in a medium, wherein a concentration of carbohydrates in the medium is greater than 20 mM; and d. fermenting the hydrolyzed biomass material under conditions and for a time sufficient to produce a fuel. - View Dependent Claims (2, 4, 5, 6, 7)
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3. The method of claim wherein the biomass material, is hydrolyzed by treatment with an acid.
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8-39. -39. (canceled)
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40. A method of making ethanol from biomass material, the method comprising:
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a. providing a biomass material comprising as high molecular weight carbohydrate; b. hydrolyzing the biomass material to provide a hydrolyzed biomass material; c. combining the hydrolyzed biomass material with Clostridium phytofermentans cells in a medium, wherein a concentration of carbohydrates in the medium is greater than 20 mM; and d. fermenting the hydrolyzed biomass material under conditions and for a time sufficient to produce ethanol. - View Dependent Claims (41, 42, 43, 44, 45)
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Specification