Nuclear reprogramming factor and induced pluripotent stem cells

• US 20100216236A1
• Filed: 02/18/2010
• Published: 08/26/2010
• Est. Priority Date: 12/13/2005
• Status: Active Grant

• Current Assignee: Kyoto University
• Original Assignee: Kyoto University
• Inventors: Yamanaka, Shinya
• Granted Patent: US 8,129,187 B2
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity
• CPC Class Codes: C12N 2501/602 Sox-2
• CPC Class Codes: C12N 2501/603 Oct-3/4
• CPC Class Codes: C12N 2501/604 Klf-4
• CPC Class Codes: C12N 2501/606 c-Myc
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2740/15043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 5/0696 Artificially induced pluripotent stem cells, e.g. iPS

Abstract

The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.

Claims

1. A method for preparing a somatic cell, which comprises:

(1) introducing the following four genes;

Oct3/4, Klf4, c-Myc and Sox2 into a somatic cell to give an induced pluripotent stem cell, and

(2) inducing differentiation of the induced pluripotent stem cell obtained in (1).

3. The method of claim 1, wherein the somatic cell is a human cell.

2. A method for preparing a somatic cell, which comprises:

(1) incubating in the presence of basic fibroblast growth factor a somatic cell in which the following three genes;

Oct3/4, Klf4 and Sox2 are introduced to give an induced pluripotent stem cell, and

(2) inducing differentiation of the induced pluripotent stem cell obtained in (1).

4. The method of claim 2, wherein the somatic cell is a human cell.