FORMING CELL STRUCTURE WITH TRANSIENT LINKER IN CAGE
First Claim
1. A method of forming a cellular structure, comprising:
- supplying cells and a transient linker to a volume partially enclosed by a cage, said linker facilitating initial attachment of adjacent cells to form a cell aggregate, said cage defining distributed openings that are sized to retain said cell aggregate;
supplying a fluid comprising a cell culture medium to said volume;
withdrawing said fluid from said volume through said openings; and
culturing aggregated cells retained in said volume to form a cell structure.
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Accused Products
Abstract
In a method of forming a cellular structure, cells and a transient linker are supplied to a volume partially enclosed by a cage. The linker facilitates initial attachment of adjacent cells to form a cell aggregate. The cage defines distributed openings that are sized to retain the cell aggregate. A fluid comprising a cell culture medium is supplied to the volume. The fluid is withdrawn from the volume through the openings. Aggregated cells retained in the volume are cultured to form a cell structure. A cell culturing device is provided which comprises a conduit and a cage in the conduit. A fluid flows in the conduit. The fluid comprises the cells, the transient linker and the cell culture medium. The cage retains aggregated cells formed in the fluid, and defines distributed openings that allow the fluid to flow through.
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Citations
30 Claims
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1. A method of forming a cellular structure, comprising:
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supplying cells and a transient linker to a volume partially enclosed by a cage, said linker facilitating initial attachment of adjacent cells to form a cell aggregate, said cage defining distributed openings that are sized to retain said cell aggregate; supplying a fluid comprising a cell culture medium to said volume; withdrawing said fluid from said volume through said openings; and culturing aggregated cells retained in said volume to form a cell structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A cell culturing device, comprising:
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a conduit; a fluid flowing in said conduit, said fluid comprising cells, a transient linker and a cell culture medium, said linker facilitating initial attachment of adjacent cells to form a cell aggregate; and a cage in said conduit for retaining aggregated cells formed in said fluid, said cage defining distributed openings that allow said fluid to flow through. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification