SAMPLE PROCESSING METHODS
First Claim
1. A method of processing a sample, comprising:
- introducing a sample into a vessel, the vessel having proximal and distal ends, the sample being introduced into the proximal end of the vessel;
incubating the sample in the vessel with a substance capable of specific binding to a preselected component of the sample;
propelling components of the incubated sample, other than the preselected component, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained;
propelling the preselected component toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and
mixing the preselected component with a reagent in the distal segment of the vessel.
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Accused Products
Abstract
A method of processing a sample may include introducing a sample into a vessel, the vessel having proximal and distal ends, the sample being introduced into the proximal end of the vessel; incubating the sample in the vessel with a substance capable of specific binding to a preselected component of the sample; propelling components of the incubated sample, other than the preselected component, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained; propelling the preselected component toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and mixing the preselected component with a reagent in the distal segment of the vessel.
112 Citations
40 Claims
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1. A method of processing a sample, comprising:
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introducing a sample into a vessel, the vessel having proximal and distal ends, the sample being introduced into the proximal end of the vessel; incubating the sample in the vessel with a substance capable of specific binding to a preselected component of the sample; propelling components of the incubated sample, other than the preselected component, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained; propelling the preselected component toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and mixing the preselected component with a reagent in the distal segment of the vessel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. A method of processing a sample, comprising:
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introducing a sample into a vessel, the vessel having proximal and distal ends and comprising a plurality of fluidly isolated segments, the sample being introduced into the proximal end of the vessel; incubating the sample in the vessel with magnetic beads capable of specific binding to nucleic acid within the sample, so that nucleic acids become bound to the magnetic beads; capturing the incubated magnetic beads; propelling components of the incubated sample, other than the captured magnetic beads, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained; washing the captured magnetic beads by propelling a wash reagent from a distal segment to be in contact with the magnetic beads within the vessel, and propelling the wash liquid towards the proximal end of the vessel by clamping the vessel distal to the magnetic beads and compressing the vessel where the magnetic beads are contained, eluting the nucleic acid from the washed magnetic beads by propelling an elution reagent from a distal segment to mix with the magnetic beads, and resuspending the magnetic beads in the elution reagent; propelling the eluted nucleic acid mixture toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and mixing the preselected component with a detection reagent in the distal segment of the vessel.
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40. A method of processing a sample, comprising:
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introducing a sample into a vessel, the vessel having proximal and distal ends and comprising a plurality of fluidly isolated segments, the sample being introduced into the proximal end of the vessel; isolating nucleic acid from the sample by incubating the sample in the vessel with at least one reagent contained within a vessel segment and propelling components of the incubated sample, other than the nucleic acid, toward the proximal end of the vessel by clamping the vessel distal to the incubated sample and compressing the vessel where the incubated sample is contained; propelling the isolated nucleic acid toward a distal segment of the vessel by clamping the vessel proximal to the preselected component and compressing the vessel where the preselected component is contained; and mixing the preselected component with a detection reagent in the distal segment of the vessel; and detecting a signal from within the vessel; wherein all steps are performed within the vessel without opening the vessel after the sample is introduced.
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Specification