APPARATUS AND METHOD OF ACQUIRING IMAGES OF A SAMPLE OF BIOLOGICAL ORIGIN
First Claim
1. An apparatus acquiring an image of a sample of biological origin with an optical image formation device, comprising an illuminating portion which illuminates the sample;
- an illumination image acquiring portion which acquires an illumination image of the sample, a luminescence image acquiring portion which acquires a luminescence image of a cell in the sample;
an image processing portion which superposes the illumination image and the luminescence image to generate a superposition image, and determines an analysis region in the superposition image.
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Abstract
A novel apparatus and a novel method of imaging a luminescence phenomenon of a sample of biological origin using an optical image formation device are provided. In the inventive method and apparatus, an illumination image acquired by illuminating the sample and a luminescence image acquired with the light emitted by a luminescence phenomenon of a cell in the sample without illuminating it are superposed to generate a superposition image. In the superposition image, an analysis region is determined. Thereby, even when the light detected in a luminescence observation is feeble, a luminescent cell and/or a luminescent site in a cell in a sample can be specified.
18 Citations
23 Claims
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1. An apparatus acquiring an image of a sample of biological origin with an optical image formation device, comprising an illuminating portion which illuminates the sample;
- an illumination image acquiring portion which acquires an illumination image of the sample, a luminescence image acquiring portion which acquires a luminescence image of a cell in the sample;
an image processing portion which superposes the illumination image and the luminescence image to generate a superposition image, and determines an analysis region in the superposition image. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- an illumination image acquiring portion which acquires an illumination image of the sample, a luminescence image acquiring portion which acquires a luminescence image of a cell in the sample;
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10. A method of acquiring an image of a sample of biological origin by means of an optical image formation device, characterized in that the method comprises the steps of:
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illuminating the sample and acquiring its illumination image; acquiring a luminescence image of light emitted by a luminescence phenomenon of a cell in the sample without illuminating the sample; superposing the illumination image and the luminescence image to generate a superposition image; and determining an analysis region in the superposition image. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. An apparatus of acquiring an image of a sample of biological origin comprising an optical microscope, a microscopic image capturing device which captures a microscopic image of the sample observed with the optical microscope, and an image processing device which takes in the microscopic image as an image data and executes image processing, wherein the image processing device comprises an image superposing portion which generates a superposition image by superposing a transmitted light image obtained by capturing an image of the sample in a transmitted light observation of the sample under the optical microscope with the microscopic image capturing device and a luminescence image obtained by capturing light emitted by a luminescence phenomenon in a cell in the sample under the optical microscope with the microscopic image capturing device;
- and an image region specifying portion which specifies an analysis region in the superposition image.
- View Dependent Claims (23)
Specification