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Droplet Actuator Devices, Systems, and Methods

  • US 20100221713A1
  • Filed: 08/24/2009
  • Published: 09/02/2010
  • Est. Priority Date: 04/18/2006
  • Status: Active Grant
First Claim
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1. A method of detecting a target nucleic acid in a sample, the method comprising:

  • (a) providing a set of nucleic acid amplification reaction droplets, each droplet comprising a portion of the sample;

    (b) treating two or more subsets of the amplification reaction droplets under conditions for amplifying the target nucleic acid to yield corresponding subsets of amplified droplets comprising amplified nucleic acid, wherein each subset of the amplification reaction droplets;

    (i) comprises one or more of the amplification reaction droplets;

    (ii) is treated under different conditions for amplifying the target nucleic acid;

    (c) preparing the amplified droplets for detection;

    (d) detecting a signal from the amplified droplets;

    (e) based on the signal, determining the amount of the amplified nucleic acid present in the amplified droplets and/or the sample.

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