DETECTION OF BORDETELLA

• US 20100221715A1
• Filed: 11/17/2009
• Published: 09/02/2010
• Est. Priority Date: 01/31/2001
• Status: Active Grant

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic), Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Salat, Ulrike, Sloan, Lynne M., Lohmann, Sabine, Patel, Robin, Cockerill, Franklin R. III
• Granted Patent: US 8,507,201 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays

Abstract

The invention provides methods to detect Bordetella pertussis and/or Bordetella parapertussis in a biological sample. Primers and probes for the differential detection of B. pertussis and B. parapertussis are provided by the invention. Articles of manufacture containing such primers and probes for detecting B. pertussis and/or B. parapertussis are further provided by the invention.

Claims

1. An article of manufacture, comprising:

a pair of IS481 primers, wherein said pair of IS481 primers comprise a first IS481 primer and a second IS481 primer, wherein said first IS481 primer comprises the sequence 5′

-CCA GTT CCT CAA GGA CGC-3′

(SEQ ID NO;

1), and wherein said second IS481 primer comprises the sequence 5′

-GAG TTC TGG TAG GTG TGA GCG TA-3′

(SEQ ID NO;

2);

a pair of IS481 probes, wherein said pair of IS481 probes comprises a first IS481 probe and a second IS481 probe, wherein said first IS481 probe comprises the sequence 5′

-CAC CGC TTT ACC CGA CCT TAC CGC CCA C-3′

(SEQ ID NO;

3), and wherein said second IS481 probe comprises the sequence 5′

-GAC CAA TGG CAA GGC TCG AAC GCT TCA TC-3′

(SEQ ID NO;

11); and

a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.

2. The article of manufacture of claim 1, wherein said first IS481 probe is labeled with said donor fluorescent moiety and wherein said second IS481 probe is labeled with said corresponding acceptor fluorescent moiety.

3. The article of manufacture of claim 1, further comprising a package insert having instructions thereon for using said pair of IS481 primers and said pair of IS481 probes to detect the presence or absence of B. pertussis in a biological sample.

7. An article of manufacture, comprising the article of manufacture of claim 1 and the article of manufacture of claim 4.

8. The article of manufacture of claim 7, further comprising a package insert having instructions thereon for using said pair of IS 481 primers, said pair of IS481 probes, said pair of IS1001 primers and said pair of IS1001 probes to distinguish between B. pertussis and B. parapertussis in a biological sample.

9. The article of manufacture of claim 7, further comprising a package insert having instructions thereon for using said pair of IS481 probes and said pair of IS1001 probes to distinguish between B. pertussis and B. parapertussis in a biological sample.

4. An article of manufacture, comprising:

a pair of IS1001 primers, wherein said pair of IS1001 primers comprise a first IS1001 primer and a second IS1001 primer, wherein said first IS1001 primer comprises the sequence 5′

-GGC GAT ATC AAC GGG TGA-3′

(SEQ ID NO;

5), and wherein said second IS1001 primer comprises the sequence 5′

-CAG GGC AAA CTC GTC CAT C-3′

(SEQ ID NO;

6);

a pair of IS1001 probes, wherein said pair of IS1001 probes comprise a first IS1001 probe and a second IS1001 probe, wherein said first IS1001 probe comprises the sequence 5′

-GGT TGG CAT ACC GTC AAG A-3′

(SEQ ID NO;

12), and wherein said second IS1001 probe comprises the sequence 5′

-GCT GGA CAA GGC TCG-3′

(SEQ ID NO;

13); and

a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.

5. The article of manufacture of claim 4, wherein said first IS1001 probe is labeled with said donor fluorescent moiety and wherein said second IS1001 probe is labeled with said corresponding acceptor fluorescent moiety.

6. The article of manufacture of claim 4, further comprising a package insert having instructions thereon for using said pair of IS1001 primers and said pair of IS1001 probes to detect the presence or absence of B. pertussis and/or B. parapertussis in a biological sample.