METHOD FOR CORRECTING A SPECTRAL IMAGE FOR OPTICAL ABERRATIONS USING SOFTWARE
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Abstract
A spectral image is corrected for optical aberrations. Tissue is exposed to a high-intensity, narrow band of light. The narrow band of light is scattered by at least one analyte in the tissue. Raman signals are optically collected from the scattered light. The Raman signals are directed to a wavelength-separating device. The Raman signals are detected as a function of intensity and wavelength to create the spectral image. The spectral image is corrected for optical aberrations using a software algorithm to spatially reassign intensity. The software may be adapted to use a reference image to make dynamic corrections. Fluorescence signals may also be collected.
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Citations
35 Claims
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1-20. -20. (canceled)
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21. A method for correcting a spectral image for optical aberrations using an instrument, the method comprising the acts of:
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exposing a sample to a high-intensity band of light; optically collecting at least one spectral image of the sample, each of the at least one image being a function of light intensity and wavelength; correcting the at least one image for optical aberrations using software to spatially reassign intensity. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29)
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30. A method for correcting a spectral image for optical aberrations using an instrument, the method comprising the acts of:
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collecting a calibration light from a known calibration light source and configuring a software algorithm based on a location of a spectral feature of the calibration light; exposing a sample to a high-intensity, narrow band of light, the narrow band of light being scattered by at least one analyte in the sample; optically collecting signals from the scattered light; directing the signals to a wavelength-separating device; detecting the signals as a function of intensity and wavelength to create at least one spectral image; and correcting the at least one spectral image for optical aberrations using the software algorithm to spatially reassign intensity. - View Dependent Claims (31, 32, 33, 34, 35)
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Specification