Affinity capture mass spectroscopy with a porous silicon biosensor
First Claim
Patent Images
1. A process for making label-free molecular ligand-analyte binding measurements comprising the steps of:
- A) providing a label-free porous silicon based optical sensor comprising;
a) a spectrometer based optical system comprising at least one light source and two spectrometers,b) at least one porous silicon region comprising at least 1000 pores, each pore having a nominal width and a nominal depth at least 10 times larger than said nominal width with the depth of at least most of the pores in said porous silicon region defining a top surface and a bottom surface being parallel or approximately parallel to the top surface, said porous silicon region being adapted to permit molecular binding interactions,c) a computer processor adapted to produce optical path difference information based on output from each of said two spectrometers,B) providing a mass spectrometer,C) depositing in a plurality of said at least 1000 pores a fluid containing a ligand chosen to attach to walls of said plurality of said at least 1000 pores,D) depositing in a said plurality of said at least 1000 pores a fluid containing a buffer and an analyte containing fluid,E) utilizing the label-free porous silicon based optical sensor to monitor the molecular binding interactions of said ligands and analytes in said analyte containing fluid deposited in said plurality of said at least 1000 pores,F) separating at least a portion of said buffer from at least a portion of said analytes and analyze with said mass spectrometer said analytes so separated at least one time increment,G) using information from said optical sensor and said mass spectrometer to characterize molecular binding and disassociation reactions between said analytes and said ligands.
2 Assignments
0 Petitions
Accused Products
Abstract
Affinity Capture-Mass Spectroscopy (AC-MS), an analytical technique which couples the sensitivity of a label-free binding detected biosensor, and the information richness of mass spectroscopy is described. A 3-dimensional porous silicon bio-surface is used to capture proteins, DNA, or small molecules while acquiring a label-free, time resolved signal linearly proportional to the amount of binding. A switch to dissociative buffer conditions then frees the captured molecule for analysis by mass spectroscopy. In particular, techniques for use with electrospray mass spectroscopy are described.
-
Citations
16 Claims
-
1. A process for making label-free molecular ligand-analyte binding measurements comprising the steps of:
-
A) providing a label-free porous silicon based optical sensor comprising; a) a spectrometer based optical system comprising at least one light source and two spectrometers, b) at least one porous silicon region comprising at least 1000 pores, each pore having a nominal width and a nominal depth at least 10 times larger than said nominal width with the depth of at least most of the pores in said porous silicon region defining a top surface and a bottom surface being parallel or approximately parallel to the top surface, said porous silicon region being adapted to permit molecular binding interactions, c) a computer processor adapted to produce optical path difference information based on output from each of said two spectrometers, B) providing a mass spectrometer, C) depositing in a plurality of said at least 1000 pores a fluid containing a ligand chosen to attach to walls of said plurality of said at least 1000 pores, D) depositing in a said plurality of said at least 1000 pores a fluid containing a buffer and an analyte containing fluid, E) utilizing the label-free porous silicon based optical sensor to monitor the molecular binding interactions of said ligands and analytes in said analyte containing fluid deposited in said plurality of said at least 1000 pores, F) separating at least a portion of said buffer from at least a portion of said analytes and analyze with said mass spectrometer said analytes so separated at least one time increment, G) using information from said optical sensor and said mass spectrometer to characterize molecular binding and disassociation reactions between said analytes and said ligands. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
Specification