Methods for the synthesis of arrays of DNA probes
First Claim
1. A method of projecting a pattern onto a surface comprising the steps of:
- (a) providing a substrate with an active surface to which protected linker molecules have been applied;
(b) projecting a two-dimensional light image onto the active surface of the substrate using projection optics comprising reflective optical elements to illuminate pixel sites on the active surface to photodeprotect the linker molecules thereon.
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Abstract
The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing reflective projection optics. The projection optics project a light image onto the active surface of the substrate to deprotect linker molecules thereon. A first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different light image, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto.
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Citations
26 Claims
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1. A method of projecting a pattern onto a surface comprising the steps of:
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(a) providing a substrate with an active surface to which protected linker molecules have been applied; (b) projecting a two-dimensional light image onto the active surface of the substrate using projection optics comprising reflective optical elements to illuminate pixel sites on the active surface to photodeprotect the linker molecules thereon. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of projecting a pattern onto a surface comprising the steps of:
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(a) providing a substrate with an active surface to which protected linker molecules have been applied; (b) projecting a two-dimensional light image onto the active surface of the substrate using projection optics comprising a concave mirror and a convex mirror, the concave mirror reflecting light to the convex mirror which reflects the light back to the concave mirror which reflects the light to the active surface where the light illuminates pixel sites on the active surface to photodeprotect the linker molecules thereon. - View Dependent Claims (23, 24, 25, 26)
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Specification