Coatings and Surface Treatments Having Active Enzymes and Peptides

• US 20100233146A1
• Filed: 05/29/2009
• Published: 09/16/2010
• Est. Priority Date: 09/09/2002
• Status: Abandoned Application

• Current Assignee: Reactive Surfaces Limited
• Original Assignee: Reactive Surfaces Limited
• Inventors: McDaniel, C. Steven
• Application Number:
  US12/474,921
• Publication Number:
  US 20100233146A1
• Time in Patent Office: Days
• US Class Current: 424/94.200
• CPC Class Codes: A01N 25/10 Macromolecular compounds
• CPC Class Codes: A01N 37/46 N-acyl derivatives
• CPC Class Codes: A01N 63/00 Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03
• CPC Class Codes: A01N 63/10 Animals; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/14 Insects
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/50 Isolated enzymes; Isolated proteins peptides A01N37/46
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C09D 5/14 Paints containing biocides, e.g. fungicides, insecticides or pesticides C09D5/16 takes precedence
• CPC Class Codes: C09D 5/1625 organic
• CPC Class Codes: C09D 7/63 organic

Abstract

Disclosed herein are a materials such as a coating, an elastomer, an adhesive, a sealant, a textile finish, a wax, and a filler for such a material, wherein the material includes an enzyme such as an esterase (e.g., a lipolytic enzyme, a sulfuric ester hydrolase, an organophosphorus compound degradation enzyme), an enzyme that degrades a cell wall and/or a cell membrane component (e.g., a lysozyme, a lytic transgrycosylase, a peptidase), and/or a biocidal or biostatic peptide. Also disclosed herein are methods of decontaminating a surface comprising such a material from a chemical substrate of an enzyme such as a lipid or an organophosphorus compound, as well as reducing the growth of a microorganism on or within such a material.

Claims

1. A composition, comprising an architectural coating, an automotive coating, a can coating, a sealant coating, a chemical agent resistant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating;

an elastomer;

an adhesive;

a sealant, a wax, a textile finish, a filler, or a combination thereof;

wherein the composition comprises an active enzyme, an antibiological peptidic agent, or a combination thereof; and

wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

2. The composition of claim 1, wherein the active enzyme comprises a plurality of active enzymes.

3. The composition of claim 1, wherein the enzyme comprises an esterase, a ceramidase, or a combination thereof, and wherein the esterase comprises a lipolytic enzyme, a phosphoric triester hydrolase, a sulfuric ester hydrolase, or a combination thereof.

4. The composition of claim 3, wherein the lipolytic enzyme, the ceramidase, or a combination thereof, comprises a carboxylesterase, a lipase, a lipoprotein lipase, an acylglycerol lipase, a hormone-sensitive lipase, a phospholipase A₁, a phospholipases A₂, a phosphatidylinositol deacylase, a phospholipase C, a phospholipase D, a phosphoinositide phospholipase C, a phosphatidate phosphatase, a lysophospholipase, a sterol esterase, a galactolipase, a sphingomyelin phosphodiesterase, a sphingomyelin phosphodiesterases D, a ceramidase, a wax-ester hydrolase, a fatty-acyl-ethyl-ester synthase, a retinyl-palmitate esterase, a 11-cis-retinyl-palmitate hydrolase, an all-trans-retinyl-palmitate hydrolase, a cutinase, an acyloxyacyl hydrolase, or a combination thereof.

5. The composition of claim 4, wherein the lipolytic enzyme, the ceramidase, or a combination thereof comprises:

a carboxylesterase derived from Actinidia deliciosa, Aedes aegypti, Aeropyrum pernix, Alicyclobacillus acidocaldarius, Aphis gossypii, Arabidopsis thaliana, Archaeoglobus fulgidus, Aspergillus clavatus, Athalia rosae, Bacillus acidocaldarius, Bombyx mandarina, Bombyx mori, Bos taurus, Burkholderia gladioli, Caenorhabditis elegans, Canis familiaris, Cavia porcellus, Chloroflexus aurantiacus, Felis catus, Fervidobacterium nodosum, Helicoverpa armigera, Homo sapiens, Macaca fascicularis, Malus pumila, Mesocricetus auratus, Mus musculus, Musca domestica, Mycoplasma hyopneumoniae, Myxococcus xanthus, Neosartorya fischeri, Oryctolagus cuniculus, Paeonia suffruticosa, Pseudomonas aeruginosa, Rattus norvegicus, Rubrobacter xylanophilus, Spodoptera exigua, Spodoptera litura, Sulfolobus acidocaldarius, Sulfolobus shibatae, Sulfolobus solfataricus, Sus scrofa, Thermotoga maritime, Thermus thermophilus, Vaccinium corymbosum, Vibrio harveyi, Xenopsylla cheopis, Yarrowia lipolytica, or a combination thereof;

a lipase derived from Acinetobacter, Aedes aegypti, Anguilla japonica, Antrodia cinnamomea, Arabidopsis rosette, Arabidopsis thaliana, Arxula adeninivorans, Aspergillus niger, Aspergillus oryzae, Aspergillus tamarii, Aureobasidium pullulans, Avena sativa, Bacillus lichenifonnis, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bombyx mandarina, Bombyx mori, Bos Taurus, Brassica napus, Brassica rapa, Burkholderia cepacia, Caenorhabditis elegans, Candida albicans, Candida antarctica, Candida deformans, Candida parapsilosis, Candida rugosa, Candida thermophila, Canis domesticus, Chenopodium rubrum, Clostridium beijerinckii, Clostridium botulinum, Clostridium novyi, Danio rerio, Galactomyces geotrichum, Gallus gallus, Geobacillus, Gibberella zeae, Gossypium hirsutum, Homo sapiens, Kurtzmanomyces sp., Leishmania infantum, Lycopersicon esculentum L, Malassezia furfur, Methanosarcina acetivorans, Mus musculus, Mus spretus, Mycobacterium tuberculosis, Mycoplasma hyopneumoniae, Myxococcus xanthus, Neosartorya fischeri, Oryctolagus cuniculus, Oryza sativa, Penicillium cyclopium, Phlebotomus papatasi, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas sp, Rattus norvegicus, Rhizomucor miehei, Rhizopus oryzae, Rhizopus stolonifer, Ricinus communis, Samia cynthia ricini, Schizosaccharomyces pombe, Serratia marcescens, Spermophilus tridecemlineatus, Staphylococcus simulans, Staphylococcus xylosus, Sulfolobus solfataricus, Sus scrofa, Thermomyces lanuginosus, Trichomonas vaginalis, Vibrio harveyi, Xenopus laevis, Yarrowia lipolytica, or a combination thereof;

a lipoprotein lipase derived from Capra hircus, Danio rerio, Felis catus, Homo sapiens, Mesocricetus auratus, Mus musculus, Oncorhynchus mykiss, Pagrus major, Papio Anubis, Rattus norvegicus, Sparus aurata, Sus scrofa, Thunnus orientalis, or a combination thereof;

an acylglycerol lipase derived from Bacillus sp., Danio rerio, Homo sapiens, Leishmania infantum, Mus musculus, Mycobacterium tuberculosis, Penicillium camembertii, Rattus norvegicus, Solanum tuberosum, or a combination thereof;

a hormone sensitive lipase derived from Bos Taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Spermophilus tridecemlineatus, Sus scrofa, Tetrahymena thermophila, or a combination thereof;

a phospholipase A₁ derived from Arabidopsis, Aspergillus oryzae, Bos Taurus, Brassica rapa, Caenorhabditis elegans, Capsicum annuum, Danio rerio, Homo sapiens, Mus musculus, Nicotiana tabacum, Polistes annularis, Polybia paulista, Rattus norvegicus, Serratia sp., Vespula vulgaris, or a combination thereof;

a phospholipase A₂ derived from Acanthaster planci, Adamsia carciniopado, Aedes aegypti, Aeropyrum pernix, Aipysurus eydouxii, Apis mellifera, Arabidopsis thaliana, Aspergillus nidulans, Austrelaps superbus, Bitis gabonica, Bos taurus, Bothriechis schlegelii, Bothrops jararacussu, BrachyDanio rerio, Bungarus caeruleus, Bungarus fasciatus, Canis familiaris, Cavia sp., Cerrophidion godmani, Chlamydomonas reinhardtii, Chrysophrys major, Crotalus viridis viridis, Daboia russellii, Danio rerio, Drosophila melanogaster, Echis carinatus, Echis ocellatus, Echis pyramidum leakeyi, Emericella nidulans, Equus caballus, Gallus gallus, Homo sapiens, Lapemis hardwickii, Laticauda semifasciata, Micrurus corallines, Mus musculus, Mytilus edulis, Naja kaouthia, Naja naja, Naja naja sputatrix, Nicotiana tabacum, Ophiophagus hannah, Ornithodoros parkeri, Oryctolagus cuniculus, Pagrus major, Patiria pectinifera, Polyandrocarpa misakiensis, Protobothrops mucrosquamatus, Rattus norvegicus, Sistrurus catenatus tergeminus, Trimeresurus borneensis, Trimeresurus flavoviridis, Trimeresurus gracilis, Trimeresurus gramineus, Trimeresurus okinavensis, Trimeresurus puniceus, Trimeresurus stejnegeri, Tuber borchii, Urticina crassicornis, Vipera russelli siamensis, Xenopus laevis, Xenopus tropicalis, or a combination thereof;

a phospholipase C derived from Aedes aegypti, Aplysia californica, Arabidopsis thaliana, Asterina miniata, Bacillus cereus, Bacillus thuringiensis, Bos taurus, Caenorhabditis elegans, Chaetopterus pergamentaceus, Chlamydomonas reinhardtii, Coturnix japonica, Danio rerio, Dictyostelium discoideum, Drosophila melanogaster, Gallus gallus, Homarus americanus, Homo sapiens, Loligo pealei, Lytechinus pictus, Meleagris gallopavo, Misgurnus mizolepis, Mus musculus, Nicotiana tabacum, Oryza sativa, Oryzias latipes, Petunia inflate, Pichia stipitis, Pisum sativum, Plasmodium falciparum, Rattus norvegicus, Strongylocentrotus purpuratus, Sus scrofa, Torenia fournieri, Toxoplasma gondii, Watasenia scintillans, Xenopus laevis, Zea mays, or a combination thereof;

a phospholipase D derived from Aedes aegypti, Arabidopsis thaliana, Arachis hypogaea, Bos taurus, Brassica oleracea, Caenorhabditis elegans, Cricetulus griseus, Cucumis melo var. inodorus, Cucumis sativus, Dictyostelium discoideum, Drosophila melanogaster, Emericella nidulans, Fragaria ananassa, Gossypium hirsutum, Homo sapiens, Lolium temulentum, Lycopersicon esculentum, Mus musculus, Oryza sativa, Papaver somniferum, Paralichthys olivaceus, Pichia stipitis, Pimpinella brachycarpa, Rattus norvegicus, Ricinus communis, Streptoverticillium cinnamoneum, Vigna unguiculata, Vitis vinifera, Zea mays, or a combination thereof;

a phosphoinositide phospholipase C derived from Arabidopsis thaliana, Aspergillus clavatus, Aspergillus fumigatus, Brassica napus, Homo sapiens, Leishmania infantum, Mus musculus, Neosartorya fischeri, Physcomitrella patens, Pichia stipitis, Rattus norvegicus, Toxoplasma gondii, Trypanosoma brucei, Vigna unguiculata, Xenopus tropicalis, Zea mays, or a combination thereof;

a phosphatidate phosphatase derived from Saccharomyces cerevisiae, or a combination thereof;

a lysophospholipase derived from Aedes aegypti, Argas monolakensis, Aspergillus clavatus, Aspergillus fumigatus, Bos Taurus, Cavia porcellus, Clonorchis sinensis, Danio rerio, Dictyostelium discoideum, Emericella nidulans, Giardia lamblia, Homo sapiens, Monodelphis domestica, Mus musculus, Neosartorya fischeri, Pichia jadinii, Pichia stipitis, Rattus norvegicus, Schistosoma japonicum, Schizosaccharomyces pombe, Sclerotinia sclerotiorum, Xenopus tropicalis, or a combination thereof;

a sterol esterase derived from Candida rugosa, Homo sapiens, Melanocarpus albomyces, Rattus norvegicus, or a combination thereof;

a galactolipase derived from Homo sapiens, Solanum tuberosum, Vigna unguiculata, or a combination thereof;

a sphingomyelin phosphodiesterase derived from Bacillus cereus, Homo sapiens, Pseudomonas sp., or a combination thereof;

a ceramidase derived from Homo sapiens, Pseudomonas, or a combination thereof;

a cutinase derived from Fusarium solani pisi, Monilinia fructicola, Pseudomonas putida, or a combination thereof;

a retinyl palmitate esterase derived from Bos Taurus;

or a combination thereof.

6. The composition of claim 5, wherein the lipolytic enzyme comprises:

a thermophilic carboxylesterase derived from Aeropyrum pernix, Alicyclobacillus acidocaldarius, Archaeoglobus fulgidus, Bacillus acidocaldarius, Pseudomonas aeruginosa, Sulfolobus shibatae, Sulfolobus solfataricus, Thermotoga maritime, or a combination thereof;

a themophilic lipase derived from Acinetobacter calcoaceticus, Acinetobacter sp., Bacillus sphaericus, Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Candida rugosa, Candida thermophila, GeoBacillus thermoleovorans Toshki, Pseudomonas fragi, Staphylococcus xylosus, Sulfolobus solfataricus, or a combination thereof;

a psychrophilic lipase derived from Pseudomonas fluorescens;

or a combination thereof;

a thermophilic phospholipase A₂ derived from Aeropyrum pernix;

a thermophilic phospholipase C derived from Bacillus cereus;

or a combination thereof.

7. The composition of claim 3, wherein the phosphoric triester hydrolase comprises an aryldialkylphosphatase, a diisopropyl-fluorophosphatase, or a combination thereof.

8. The composition of claim 7, wherein the aryldialkylphosphatase comprises an organophosphorus hydrolase, a human paraoxonase, an animal carboxylase, or a combination thereof;

wherein the diisopropyl-fluorophosphatase comprises an organophosphorus acid anhydrolase, a squid-type DFPase, a Mazur-type DFPase, or a combination thereof;

or a combination thereof of the forgoing.

9. The composition of claim 8, wherein the organophosphorus hydrolase comprises an Agrobacterium radiobacter P230 organophosphate hydrolase, a Flavobacterium balustinum parathion hydrolase, a Pseudomonas diminuta phosphotriesterase, a Flavobacterium sp opd gene product, a Flavobacterium sp. parathion hydrolase opd gene product, or a combination thereof;

wherein the animal carboxylase comprises an insect carboxylase;

or a combination thereof;

wherein the organophosphorus acid anhydrolase comprises an Altermonas organophosphorus acid anhydrolase, a prolidase, or a combination thereof;

wherein the squid-type DFPase comprises a Loligo vulgaris DFPase, a Loligo pealei DFPase, a Loligo opalescens DFPase, or a combination thereof;

wherein the Mazur-type DFPase comprises a mouse liver DFPase, a hog kidney DFPase, a Bacillus stearothermophilus strain OT DFPase, an Escherichia coli DFPase, or a combination thereof;

or a combination thereof the forgoing.

10. The composition of claim 8, wherein the insect carboxylase comprises a Podia interpunctella carboxylase, Chrysomya putoria carboxylase, Lucilia cuprina carboxylase, Musca domestica carboxylase, or a combination thereof;

wherein the Altermonas organophosphorus acid anhydrolase comprises an Alteromonas sp JD6.5 organophosphorus acid anhydrolase, an Alteromonas haloplanktis organophosphorus acid anhydrolase, an Altermonas undina organophosphorus acid anhydrolase, or a combination thereof;

wherein the prolidase comprises a human prolidase, a Mus musculus prolidase, a Lactobacillus helveticus prolidase, an Escherichia coli prolidase, an Escherichia coli aminopeptidase P, or a combination thereof;

wherein the phosphoric triester hydrolase comprises a Plesiomonas sp. strain M6 mpd gene product, a Xanthomonas sp. phosphoric triester hydrolase, a Tetrahymena phosphoric triester hydrolase, or a combination thereof;

or a combination thereof the forgoing.

11. The composition of claim 3, wherein the sulfuric ester hydrolase comprises an arylsulfatase.

12. The composition of claim 1, wherein the peptidase comprises a trypsin, a chymotrypsin, or a combination thereof.

13. The composition of claim 1, wherein the antibiological enzyme comprises a lysozyme, a lysostaphin, a libiase, a lysyl endopeptidase, a mutanolysin, a cellulase, a chitinase, an α

-agarase, an β

-agarase, a N-acetylmuramoyl-L-alanine amidase, a lytic transglycosylase, a glucan endo-1,3-β

-D-glucosidase, an endo-1,3(4)-β

-glucanase, a β

-lytic metalloendopeptidase, a 3-deoxy-2-octulosonidase, a peptide-N4-(N-acetyl-β

-glucosaminyl)asparagine amidase, a mannosyl-glycoprotein endo-β

-N-acetylglucosaminidase, a l-carrageenase, a κ

-carrageenase, a λ

-carrageenase, an α

-neoagaro-oligosaccharide hydrolase, an endolysin, an autolysin, a mannoprotein protease, a glucanase, a mannase, a zymolase, a lyticase, a lipolytic enzyme, a peroxidase, or a combination thereof.

14. The composition of claim 1, wherein the antibiological peptidic agent comprises SEQ ID No. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, or a combination thereof.

15. The composition of claim 1, wherein the antibiological peptidic agent comprises a plurality of antibiological peptidic agents.

16. The composition of claim 1, wherein the active enzyme comprises a mesophilic enzyme, a psychrophilic enzyme, a thermophilic enzyme, a halophilic enzyme, or a combination thereof.

17. The composition of claim 1, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, comprises an immobilization carrier.

18. The composition of claim 1, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, comprises a purified active enzyme, a purified antibiological peptidic agent, or a combination thereof.

19. The composition of claim 1, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, comprises a particulate material.

20. The composition of claim 19, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, comprises a cell-based particulate material.

21. The composition of claim 20, wherein the cell-based particulate material comprises a whole cell particulate material or a cell fragment particulate material.

22. The composition of claim 19, wherein the average wet molecular weight or dry molecular weight of a primary particle of the particulate material is about 50 kDa to about 1.5×

10¹⁴ kDa.

23. The composition of claim 19, wherein an average active enzyme content, an average antibiological peptidic agent content, or a combination thereof, per primary particle of the particulate material is about 0.01% to about 100%.

24. The composition of claim 1, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, is attenuated, sterilized, or a combination thereof.

25. The composition of claim 1, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, comprises about 0.01% to about 80% of the composition by weight or volume.

26. The composition of claim 1, wherein the active enzyme, the antibiological peptidic agent, or a combination thereof, is microencapsulated.

27. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, is about 5 um to about 5000 um thick upon a surface.

28. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a paint.

29. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a clear coating.

30. The composition of claim 29, wherein the clear coating comprises a lacquer, a varnish, a shellac, a stain, a water repellent coating, or a combination thereof.

31. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a multicoat system.

32. The composition of claim 31, wherein the multicoat system comprises 2 to 10 layers.

33. The composition of claim 31, wherein a plurality of layers of the multicoat system comprise the active enzyme.

34. The composition of claim 31, wherein the multicoat system comprises a sealer, a water repellent, a primer, an undercoat, a topcoat, or a combination thereof.

35. The composition of claim 34, wherein the topcoat comprises the active enzyme.

36. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a coating that is capable of film formation.

37. The composition of claim 36, wherein film formation occurs between about −

10°

C. to about 40°

C.

38. The composition of claim 36, wherein film formation occurs at baking conditions.

39. The composition of claim 36, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a volatile component and a non-volatile component, and wherein film formation occurs by loss of part of the volatile component.

40. The composition of claim 36, wherein film formation occurs by cross-linking of a binder.

41. The composition of claim 36, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, produces a self-cleaning film.

42. The composition of claim 36, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, produces a temporary film.

43. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a non-film forming coating.

44. The composition of claim 43, wherein the non-film forming coating comprises a non-film formation binder.

45. The composition of claim 43, wherein the non-film forming coating comprises a coating component in a concentration that is insufficient to produce a solid film.

46. The composition of claim 1, wherein the architectural coating comprises an architectural wood coating, an architectural masonry coating, an architectural artist'"'"'s coating, an architectural plastic coating, an architectural metal coating, or a combination thereof.

47. The composition of claim 1, wherein the architectural coating has a pot life of at least 12 months at about −

10°

C. to about 40°

C.

48. The composition of claim 1, wherein the composition comprises an automotive coating, a can coating, a sealant coating, or a combination thereof.

49. The composition of claim 1, wherein the composition comprises a chemical agent resistant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating, or a combination thereof.

50. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a coating for a plastic surface.

51. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a water-borne coating.

52. The composition of claim 51, wherein the water-borne coating comprises a latex coating.

53. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a solvent-borne coating.

54. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, has a low-shear viscosity of about 100 P to about 3000 P, has a medium-shear viscosity of about 84 Ku and about 140 Ku, has a high-shear viscosity of about 0.5 P to about 2.5 P, or a combination thereof.

55. The composition of claim 1, wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, comprises a binder, a liquid component, a colorant, an additive, or a combination thereof.

56. The composition of claim 55, wherein the binder comprises a thermoplastic binder, a thermosetting binder, or a combination thereof.

57. The composition of claim 55, wherein the binder comprises an oil-based binder, a polyester resin, a modified cellulose, a polyamide, an amino resin, a urethane binder, a phenolic resin, an epoxy resin, a polyhydroxyether binder, an acrylic resin, a polyvinyl binder, a rubber resin, a bituminous binder, a polysulfide binder, a silicone binder, an organic binder, or a combination thereof.

58. The composition of claim 57, wherein the oil-based binder comprises an oil, an alkyd, an oleoresinous binder, a fatty acid epoxide ester, or a combination thereof;

wherein the polyester resin comprises a hydroxy-terminated polyester, a carboxylic acid-terminated polyester, or a combination thereof;

wherein the modified cellulose comprises a cellulose ester, a nitrocellulose, or a combination thereof;

wherein the epoxy resin comprises a cycloaliphatic epoxy binder;

wherein the rubber resin comprises a chlorinated rubber resin, a synthetic rubber resin, or a combination thereof;

or a combination thereof the forgoing.

59. The composition of claim 55, wherein the liquid component comprises a solvent, a thinner, a diluent, a plasticizer, or a combination thereof.

60. The composition of claim 55, wherein the liquid component comprises a liquid organic compound, an inorganic compound, water, or a combination thereof.

61. The composition of claim 60, wherein the liquid organic compound comprises a hydrocarbon, an oxygenated compound, a chlorinated hydrocarbon, a nitrated hydrocarbon, a miscellaneous organic liquid, a plasticizer, or a combination thereof;

wherein the inorganic compound comprises ammonia, hydrogen cyanide, hydrogen fluoride, hydrogen cyanide, sulfur dioxide, or a combination thereof;

wherein the water comprises methanol, ethanol, propanol, isopropyl alcohol, tert-butanol, ethylene glycol, methyl glycol, ethyl glycol, propyl glycol, butyl glycol, ethyl diglycol, methoxypropanol, methyldipropylene glycol, dioxane, tetrahydrorfuran, acetone, diacetone alcohol, dimethylformamide, dimethyl sulfoxide, ethylbenzene, tetrachloroethylene, p-xylene, toluene, diisobutyl ketone, tricholorethylene, trimethylcyclohexanol, cyclohexyl acetate, dibutyl ether, trimethylcyclohexanone, 1,1,1-tricholoroethane, hexane, hexanol, isobutyl acetate, butyl acetate, isophorone, nitropropane, butyl glycol acetate, 2-nitropropane, methylene chloride, methyl isobutyl ketone, cyclohexanone, isopropyl acetate, methylbenzyl alcohol, cyclohexanol, nitroethane, methyl tert-butyl ether, ethyl acetate, diethyl ether, butanol, butyl glycolate, isobutanol, 2-butanol, propylene carbonate, ethyl glycol acetate, methyl acetate, methyl ethyl ketone, or a combination thereof;

or a combination thereof the forgoing.

62. The composition of claim 61, wherein the hydrocarbon comprises an aliphatic hydrocarbon, a cycloaliphatic hydrocarbon, a terpene, an aromatic hydrocarbon, or a combination thereof;

wherein the oxygenated compound comprises an alcohol, an ester, a glycol ether, a ketone, an ether, or a combination thereof;

or a combination thereof the forgoing.

63. The composition of claim 61, wherein the hydrocarbon comprises a petroleum ether, pentane, hexane, heptane, isododecane, a kerosene, a mineral spirit, a VMP naphtha, cyclohexane, methylcyclohexane, ethylcyclohexane, tetrahydronaphthalene, decahydronaphthalene, wood terpentine oil, pine oil, α

-pinene, β

-pinene, dipentene, D-limonene, benzene, toluene, ethylbenzene, xylene, cumene, a type I high flash aromatic naphtha, a type II high flash aromatic naphtha, mesitylene, pseudocumene, cymol, styrene, or a combination thereof;

wherein the oxygenated compound comprises methanol, ethanol, propanol, isopropanol, 1-butanol, isobutanol, 2-butanol, tert-butanol, amyl alcohol, isoamyl alcohol, hexanol, methylisobutylcarbinol, 2-ethylbutanol, isooctyl alcohol, 2-ethylhexanol, isodecanol, cylcohexanol, methylcyclohexanol, trimethylcyclohexanol, benzyl alcohol, methylbenzyl alcohol, furfuryl alcohol, tetrahydrofurfuryl alcohol, diacetone alcohol, trimethylcyclohexanol, methyl formate, ethyl formate, butyl formate, isobutyl formate, methyl acetate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, sec-butyl acetate, amyl acetate, isoamyl acetate, hexyl acetate, cyclohexyl acetate, benzyl acetate, methyl glycol acetate, ethyl glycol acetate, butyl glycol acetate, ethyl diglycol acetate, butyl diglycol acetate, 1-methoxypropyl acetate, ethoxypropyl acetate, 3-methoxybutyl acetate, ethyl 3-ethoxypropionate, isobutyl isobutyrate, ethyl lactate, butyl lactate, butyl glycolate, dimethyl adipate, glutarate, succinate, ethylene carbonate, propylene carbonate, butyrolactone, methyl glycol, ethyl glycol, propyl glycol, isopropyl glycol, butyl glycol, methyl diglycol, ethyl diglycol, butyl diglycol, ethyl triglycol, butyl triglycol, diethylene glycol dimethyl ether, methoxypropanol, isobutoxypropanol, isobutyl glycol, propylene glycol monoethyl ether, 1-isopropoxy-2-propanol, propylene glycol mono-n-propyl ether, propylene glycol n-butyl ether, methyl dipropylene glycol, methoxybutanol, acetone, methyl ethyl ketone, methyl propyl ketone, methyl isopropyl ketone, methyl butyl ketone, methyl isobutyl ketone, methyl amyl ketone, methyl isoamyl ketone, diethyl ketone, ethyl amyl ketone, dipropyl ketone, diisopropyl ketone, cyclohexanone, methylcylcohexanone, trimethylcyclohexanone, mesityl oxide, diisobutyl ketone, isophorone, diethyl ether, diisopropyl ether, dibutyl ether, di-sec-butyl ether, methyl tert-butyl ether, tetrahydrofuran, 1,4-dioxane, metadioxane, or a combination thereof;

wherein the chlorinated hydrocarbon comprises methylene chloride, trichloromethane, tetrachloromethane, ethyl chloride, isopropyl chloride, 1,2-dichloroethane, 1,1,1-trichloroethane, trichloroethylene, 1,1,2,2-tetrachlorethane, 1,2-dichloroethylene, perchloroethylene, 1,2-dichloropropane, chlorobenzene, or a combination thereof;

wherein the nitrated hydrocarbon comprises a nitroparaffin, N-methyl-2-pyrrolidone, or a combination thereof;

wherein the miscellaneous organic liquid comprises carbon dioxide;

acetic acid, methylal, dimethylacetal, N,N-dimethylformamide, N,N-dimethylacetamide, dimethylsulfoxide, tetramethylene suflone, carbon disulfide, 2-nitropropane, N-methylpyrrolidone, hexamethylphosphoric triamide, 1,3-dimethyl-2-imidazolidinone, or a combination thereof;

wherein the plasticizer comprises di(2-ethylhexyl) azelate;

di(butyl) sebacate;

di(2-ethylhexyl) phthalate;

di(isononyl) phthalate;

dibutyl phthalate;

butyl benzyl phthalate;

di(isooctyl) phthalate;

di(idodecyl) phthalate;

tris(2-ethylhexyl) trimellitate;

tris(isononyl) trimellitate;

di(2-ethylhexyl) adipate;

di(isononyl) adipate;

acetyl tri-n-butyl citrate;

an epoxy modified soybean oil;

2-ethylhexyl epoxytallate;

isodecyl diphenyl phosphate;

tricresyl phosphate;

isodecyl diphenyl phosphate;

tri-2-ethylhexyl phosphate;

an adipic acid polyester;

an azelaic acid polyester;

a bisphenoxyethylformal, or a combination thereof;

or a combination thereof the forgoing.

64. The composition of claim 63, wherein the colorant comprises a pigment, a dye, or a combination thereof.

65. The composition of claim 64, wherein the active enzyme comprises a particulate material comprising about 0.000001% to about 100% of the pigment.

66. The composition of claim 64, wherein the pigment volume concentration of wherein the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof, is about 20% to about 70%.

67. The composition of claim 64, wherein the pigment comprises a corrosion resistance pigment, a camouflage pigment, a color property pigment, an extender pigment, or a combination thereof.

68. The composition of claim 67, wherein the corrosion resistance pigment comprises aluminum flake, aluminum triphosphate, aluminum zinc phosphate, ammonium chromate, barium borosilicate, barium chromate, barium metaborate, basic calcium zinc molybdate, basic carbonate white lead, basic lead silicate, basic lead silicochromate, basic lead silicosulfate, basic zinc molybdate, basic zinc molybdate-phosphate, basic zinc molybdenum phosphate, basic zinc phosphate hydrate, bronze flake, calcium barium phosphosilicate, calcium borosilicate, calcium chromate, calcium plumbate, calcium strontium phosphosilicate, calcium strontium zinc phosphosilicate, dibasic lead phosphite, lead chromosilicate, lead cyanamide, lead suboxide, lead sulfate, mica, micaceous iron oxide, red lead, steel flake, strontium borosilicate, strontium chromate, tribasic lead phophosilicate, zinc borate, zinc borosilicate, zinc chromate, zinc dust, zinc hydroxy phosphite, zinc molybdate, zinc oxide, zinc phosphate, zinc potassium chromate, zinc silicophosphate hydrate, zinc tetraoxylchromate, or a combination thereof;

wherein the camouflage pigment comprises an anthraquinone black, a chromium oxide green, the active enzyme comprising a particulate material, or a combination thereof;

wherein the color property pigment comprises a black pigment, a brown pigment, a white pigment, a pearlescent pigment, a violet pigment, a blue pigment, a green pigment, a yellow pigment, an orange pigment, a red pigment, a metallic pigment, the active enzyme comprising a particulate material, or a combination thereof;

wherein the extender pigment comprises a barium sulphate, a calcium carbonate, a kaolin, a calcium sulphate, a silicate, a silica, an alumina trihydrate, an active enzyme comprising a particulate material, or a combination thereof;

or a combination thereof the forgoing.

69. The composition of claim 67, wherein the color property pigment comprises aniline black;

anthraquinone black;

carbon black;

copper carbonate;

graphite;

iron oxide;

micaceous iron oxide;

manganese dioxide, azo condensation, metal complex brown;

antimony oxide;

basic lead carbonate;

lithopone;

titanium dioxide;

white lead;

zinc oxide;

zinc sulphide;

titanium dioxide and ferric oxide covered mica, bismuth oxychloride crystal, dioxazine violet, carbazole Blue;

cobalt blue;

indanthrone;

phthalocyanine blue;

Prussian blue;

ultramarine;

chrome green;

hydrated chromium oxide;

phthalocyanine green;

anthrapyrimidine;

arylamide yellow;

barium chromate;

benzimidazolone yellow;

bismuth vanadate;

cadmium sulfide yellow;

complex inorganic color;

diarylide yellow;

disazo condensation;

flavanthrone;

isoindoline;

isoindolinone;

lead chromate;

nickel azo yellow;

organic metal complex;

yellow iron oxide;

zinc chromate;

perinone orange;

pyrazolone orange;

anthraquinone;

benzimidazolone;

BON arylamide;

cadmium red;

cadmium selenide;

chrome red;

dibromanthrone;

diketopyrrolo-pyrrole;

lead molybdate;

perylene;

pyranthrone;

quinacridone;

quinophthalone;

red iron oxide;

red lead;

toluidine red;

tonor;

β

-naphthol red;

aluminum flake;

aluminum non-leafing, gold bronze flake, zinc dust, stainless steel flake, nickel flake, nickel powder, barium ferrite;

borosilicate;

burnt sienna;

burnt umber;

calcium ferrite;

cerium;

chrome orange;

chrome yellow;

chromium phosphate;

cobalt-containing iron oxide;

fast chrome green;

gold bronze powder;

luminescent;

magnetic;

molybdate orange;

molybdate red;

oxazine;

oxysulfide;

polycyclic;

raw sienna;

surface modified pigment;

thiazine;

thioindigo;

transparent cobalt blue;

transparent cobalt green;

transparent iron blue;

transparent zinc oxide;

triarylcarbonium;

zinc cyanamide;

zinc ferrite;

or a combination thereof.

70. The composition of claim 55, wherein the additive comprises 0.000001% to 20.0% by weight, of the architectural coating, the automotive coating, the can coating, the sealant coating, the chemical agent resistant coating, the camouflage coating, the pipeline coating, the traffic marker coating, the aircraft coating, the nuclear power plant coating, or a combination thereof.

71. The composition of claim 55, wherein the additive comprises an accelerator, an adhesion promoter, an antifoamer, anti-insect additive, an antioxidant, an antiskinning agent, a buffer, a catalyst, a coalescing agent, a corrosion inhibitor, a defoamer, a dehydrator, a dispersant, a drier, electrical additive, an emulsifier, a filler, a flame/fire retardant, a flatting agent, a flow control agent, a gloss aid, a leveling agent, a marproofing agent, a preservative, a silicone additive, a slip agent, a surfactant, a light stabilizer, a rheological control agent, a wetting additive, a cryopreservative, a xeroprotectant, a pH indicator, or a combination thereof.

72. The composition of claim 71, wherein the preservative comprises an in-can preservative, an in-film preservative, or a combination thereof.

73. The composition of claim 72, wherein the preservative comprises a biocide, a biostatic, or a combination thereof.

74. The composition of claim 73, wherein the biocide, the biostatic, or a combination thereof comprises an algaecide, an algaestatic, a bactericide, a bacteristatic, a fungicide, a fungistatic, a germicide, a germistatic, a herbicide, a herbistatic, a microbiocide, a microbiostatic, a mildewcide, a mildewstatic, a molluskicide, a molluskistatic, a slimicide, a slimistatic, a viricide, a viristatic, or a combination thereof.

75. The composition of claim 71, wherein the preservative comprises 1-(3-chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride;

1,2-benzisothiazoline-3-one;

1,2-dibromo-2,4-dicyanobutane;

1,3-bis(hydroxymethyl)-5,5-dimethylhydantoin;

1-methyl-3,5,7-triaza-1-azonia-adamantane chloride;

2-bromo-2-nitropropane-1,3-diol;

2-(4-thiazolyl)benzimidazole;

2-(hydroxymethyl)-amino-2-methyl-1-propanol;

2(hydroxymethyl)-aminoethanol;

2,2-dibromo-3-nitrilopropionamide;

2,4,5,6-tetrachloro-isophthalonitrile;

2-mercaptobenzo-thiazole;

2-methyl-4-isothiazolin-3-one;

2-n-octyl-4-isothiazoline-3-one;

3-iodo-2-propynl N-butyl carbamate;

4,5-dichloro-2-N-octyl-3(2H)-isothiazolone;

4,4-dimethyloxazolidine;

5-chloro-2-methyl-4-isothiazolin-3-one;

5-hydroxy-methyl-1-aza-3,7-dioxabicylco (3.3.0.) octane;

6-acetoxy-2,4-dimethyl-1,3-dioxane;

7-ethyl bicyclooxazolidine;

a combination of 1,2-benzisothiazoline-3-one and hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine;

a combination of 1,2-benzisothiazoline-3-one and zinc pyrithione;

a combination of 2-(thiocyanomethyl-thio)benzothiozole and methylene bis(thiocyanate);

a combination of 4-(2-nitrobutyl)-morpholine and 4,4′

-(2-ethylnitrotrimethylene) dimorpholine;

a combination of 4,4-dimethyl-oxazolidine and 3,4,4-trimethyloxazolidine;

a combination of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one;

a combination of carbendazim and 3-iodo-2-propynl N-butyl carbamate;

a combination of carbendazim, 3-iodo-2-propynl N-butyl carbamate and diuron;

a combination of chlorothalonil and 3-iodo-2-propynl N-butyl carbamate;

a combination of chlorothalonil and a triazine compound;

a combination of tributyltin benzoate and alkylamine hydrochlorides;

a combination of zinc-dimethyldithiocarbamate and zinc 2-mercaptobenzothiazole;

a copper soap;

a metal soap;

a mercury soap;

a mixture of bicyclic oxazolidines;

a tin soap;

an alkylamine hydrochloride;

an amine reaction product;

barium metaborate;

butyl parahydroxybenzoate;

carbendazim;

copper(II) 8-quinolinolate;

diiodomethyl-p-tolysulfone;

dithio-2, 2-bis(benzmethylamide);

diuron;

ethyl parahydroxybenzoate;

glutaraldehyde;

hexahydro-1,3,5-triethyl-s-triazine;

hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine;

hydroxymethyl-5,5-dimethylhydantoin;

methyl parahydroxybenzoate;

N-butyl-1,2-benzisothiazolin-3-one;

N-(trichloromethylthio) phthalimide;

N-cyclopropyl-N-(1-dimethylethyl)-6-(methylthio)-1,3,5-triazine-2,4-diamine;

N-trichloromethyl-thio-4-cyclohexene-1,2-dicarboximide;

p-chloro-m-cresol;

phenoxyethanol;

phenylmercuric acetate;

poly(hexamethylene biguanide) hydrochloride;

potassium dimethyldithiocarbamate;

potassium N-hydroxy-methyl-N-methyl-dithiocarbamate;

propyl parahydroxybenzoate;

sodium 2-pyridinethiol-1-oxide;

tetra-hydro-3,5-di-methyl-2H-1,3,5-thiadiazine-2-thione;

tributyltin benzoate;

tributyltin oxide;

tributyltin salicylate;

zinc pyrithione;

sodium pyrithione;

copper pyrithione;

zinc oxide;

a zinc soap;

or a combination thereof.

76. The composition of claim 1, wherein the elastomer comprises a thermoplastic elastomer, a melt processable rubber, a synthetic rubber, a natural rubber, a propylene oxide elastomer, an ethylene-isoprene elastomer, an ethylene-vinyl acetate elastomer, a non-polymeric elastomer, or a combination thereof.

77. The composition of claim 76, wherein the thermoplastic elastomer comprises an elastomeric polyolefin, a thermoplastic vulcanizate, a styrenic thermoplastic elastomer, a styrene butadiene rubber, a polyurethane elastomer, a thermoplastic copolyester elastomer, a polyamide, or a combination thereof;

wherein the synthetic rubber comprises a nitrile butadiene rubber, a butadiene rubber, a butyl rubber, a chlorinated/chlorosulfonated polyethylene, an epichlorohydrin, an ethylene propylene copolymer, a fluoroelastomer, a polyacrylate rubber, a poly(ethylene acrylic), a polychloroprene, a polyisoprene, a polysulfide rubber, a silicone rubber, or a combination thereof;

wherein the non-polymeric elastomer comprises a vulcanized oil;

or a combination thereof.

78. The composition of claim 1, wherein the composition comprises an adhesive, a sealant, or a combination thereof.

79. The composition of claim 78, wherein the adhesive, the sealant, or a combination thereof;

comprises an acrylic adhesive, an acrylic acid diester adhesive, a butyl rubber adhesive, a carbohydrate adhesive, a cellulosic adhesive, a cyanoacrylate adhesive, a cyanate ester adhesive, an epoxy adhesive, a melamine formaldehyde adhesive, a natural rubber adhesive, a neoprene rubber adhesive, a nitrile rubber adhesive, a phenolic adhesive, a phenoxy adhesive, a polyamide adhesive, a polybenzimidazole adhesive, a polyethylene adhesive, a polyester adhesive, a polyisobutylene adhesive, a polysulfide adhesive, a polyurethane adhesive, a polyvinyl acetal adhesive, a polyvinyl acetate adhesive, a polyvinyl alcohol adhesive, a protein adhesive, a reclaimed rubber adhesive, a resorcinol adhesive, a silicone adhesive, a styrene butadiene adhesive, an urea formaldehyde adhesive, a vinyl vinylidene adhesive, a non-polymeric adhesive, or a combination thereof.

80. The composition of claim 79, wherein the non-polymeric adhesive comprises a mucilage adhesive.

81. The composition of claim 1, wherein the elastomer;

the adhesive;

the sealant, or a combination thereof, comprises a polymeric material additive.

82. The composition of claim 81, wherein the polymeric material additive comprises a curing agent, a cross-linking agent, an inhibitor, a nucleating agent, a plasticizer, a lubricant, a mold release agent, a slip agent, a diluent, a dispersant, a thickening agent, a thixotropic, a thinner, an anti-blocking agent, an antistatic agent, a flame retarder, a colorant, an antifogging agent, an odorant, a blowing agent, a surfactant, a defoamer, an anti-aging additive, a degrading agent, an anti-microbial agent, an adhesion promoter, an impact modifier, a low-profile additive, a filler, a pH indicator, or a combination thereof.

83. The composition of claim 82, wherein the anti-microbial agent comprises a biocide, a biostatic, or a combination thereof.

84. The composition of claim 1, wherein the antibiological peptidic agent, the antibiological enzyme, or a combination thereof comprises a biocide, a biostatic, or a combination thereof.

85. The composition of claim 1, wherein the composition is stored in a multi-pack container.

86. The composition of claim 85, wherein about 0.000001% to about 100% of the active enzyme, the antibiological agent, or a combination thereof, is stored in a container of the multi-pack composition, and at least one composition component is stored in another container of the multi-pack.

87. A coating composition, comprising an architectural coating comprising an active enzyme, an antibiological peptidic agent, or a combination thereof, wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

88. A multi-pack coating composition, comprising a plurality of containers, wherein at least one container comprises an active enzyme, an antibiological peptidic agent, or a combination thereof;

wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof; and

wherein the coating comprises an architectural wood coating, an architectural masonry coating, an architectural artist coating, an automotive coating, a can coating, a sealant coating, a chemical agent resistant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating, or a combination thereof.

89. An elastomer composition, comprising an elastomer and an active enzyme, an antibiological peptidic agent, or a combination thereof;

and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

90. A filler composition, comprising a filler and an active enzyme, an antibiological peptidic agent, or a combination thereof;

and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

91. An adhesive composition, comprising an adhesive and an active enzyme, an antibiological peptidic agent, or a combination thereof;

and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

92. A sealant composition, comprising a sealant and an active enzyme, an antibiological peptidic agent, or a combination thereof;

and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

93. A textile finish composition, comprising a textile finish and an active enzyme, an antibiological peptidic agent, or a combination thereof;

and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

94. A wax composition, comprising a wax and an active enzyme, an antibiological peptidic agent, or a combination thereof;

and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

95. A method of preparing a bioactive surface treatment, a bioactive filler, or a combination thereof, comprising the steps of:

obtaining an active enzyme, an antibiological peptidic agent, or a combination thereof;

wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof; and

admixing at least one component of a surface treatment, a filler, or a combination thereof, with the active enzyme, the antibiological peptidic agent, or a combination thereof; and

then admixing any additional component of a surface treatment, a filler, or a combination thereof to complete the surface treatment, the filler, or a combination thereof.

96. A method of preparing a bioactive surface treatment, a bioactive filler, or a combination thereof, comprising the steps of:

obtaining an active enzyme, an antibiological peptidic agent, or a combination thereof;

wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof; and

admixing a surface treatment, a filler, or a combination thereof, with the active enzyme, the antibiological peptidic agent, or a combination thereof.

97. A method of reducing the concentration of a chemical on a surface, comprising the steps of:

applying a surface treatment to the surface, wherein the surface treatment comprises an active enzyme, and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof; and

contacting the surface with a chemical, wherein the chemical comprises a substrate of the active enzyme; and

wherein the substrate comprises an ester linkage, a peptide linkage, a lipid, a cell wall component, a cell membrane component, or a combination thereof.

98. The method of claim 97, wherein the surface treatment comprises an architectural coating, an automotive coating, a can coating, a sealant coating, a chemical agent resistant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating;

an elastomer;

an adhesive;

a sealant, a wax, a textile finish, or a combination thereof.

99. The method of claim 97, wherein the substrate is a component of a living cell, a virus, or a combination thereof, and wherein the active enzyme produces a biocidal activity, a biostatic activity, or a combination thereof upon contact with the substrate.

100. A method of cleaning a surface contaminated with a chemical, comprising the steps of:

contacting a surface contaminated with a chemical with a coating comprising an active enzyme, wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof, wherein the chemical comprises a substrate of the active enzyme; and

wherein the substrate comprises an ester linkage, a peptide linkage, a lipid, a cell wall component, a cell membrane component, or a combination thereof.

101. A method of reducing the concentration of a chemical on a surface, comprising the steps of:

applying a coating to the surface, wherein the coating comprises an architectural wood coating, an architectural masonry coating, an architectural artist coating, an automotive coating, a can coating, a sealant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating, or a combination thereof, and wherein the coating comprises an active enzyme, wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof, and contacting the surface with a chemical, wherein the chemical comprises a substrate of the active enzyme; and

wherein the chemical comprises an ester linkage, a peptide linkage, a lipid, a cell wall component, a cell membrane component, or a combination thereof.

102. The method of claim 101, wherein the step of applying to the surface a coating occurs prior to contacting the surface with the chemical.

103. The method of claim 101, wherein the surface is located on a stove, a sink, a drain pipe, a counter top, a floor, a wall, a cabinet, an appliance, or a combination thereof.

104. The method of claim 101, wherein the coating is formulated as an interior coating.

105. The method of claim 101, further comprising the step of:

applying a cleaning material to the surface, and removing the chemical, a product of the reaction of the chemical catalyzed by the active enzyme, or a combination thereof.

106. The method of claim 105, wherein the cleaning material comprises a cleaning solution, a cleaning devise, or a combination thereof.

107. A method of cleaning a surface contaminated with a chemical, comprising the steps of:

obtaining a surface treatment comprising an active enzyme; and

contacting a surface contaminated with a chemical with the surface treatment comprising an active enzyme, wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof, wherein the chemical comprises a substrate of the active enzyme; and

wherein the chemical comprises an ester linkage, a peptide linkage, a lipid, a cell wall component, a cell membrane component, or a combination thereof.

108. A kit having component parts capable of being assembled comprising a container comprising an active enzyme, an antibiological peptidic agent, or a combination thereof, and a container comprising at least one component of an architectural coating, an automotive coating, a can coating, a sealant coating, a chemical agent resistant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating;

an elastomer;

an adhesive;

a sealant, a wax, a textile finish, a filler, or a combination thereof;

wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.

109. An article of manufacture, comprising an architectural coating, an automotive coating, a can coating, a sealant coating, a chemical agent resistant coating, a camouflage coating, a pipeline coating, a traffic marker coating, an aircraft coating, a nuclear power plant coating;

an elastomer;

an adhesive;

a sealant, a wax, a textile finish, a filler, or a combination thereof;

wherein the article of manufacture comprises an active enzyme, an antibiological peptidic agent, or a combination thereof, and wherein the active enzyme comprises an esterase, a petroleum lipolytic enzyme, a ceramidase, a peptidase, an antibiological enzyme, or a combination thereof.