Templated islet cells and small islet cell clusters for diabetes treatment
First Claim
Patent Images
1. An implantable device comprising:
- a substantially planar scaffold comprised of a biomaterial having a major surface; and
individual islet cells or small islet cell clusters attached to said surface of said biomaterial scaffold to form a multilayer of islet cells, said individual islet cells or small islet cell clusters being derived from adult intact islets, wherein said small islet cell clusters have a diameter of less than 125 μ
m.
1 Assignment
0 Petitions
Accused Products
Abstract
A scaffold having islet cells or small islet cell clusters attached thereto in a multilayer, and a micro-mold having divots for culturing islets, wherein islet formation is influenced by the shape and dimensions of the divots are disclosed.
25 Citations
25 Claims
-
1. An implantable device comprising:
-
a substantially planar scaffold comprised of a biomaterial having a major surface; and individual islet cells or small islet cell clusters attached to said surface of said biomaterial scaffold to form a multilayer of islet cells, said individual islet cells or small islet cell clusters being derived from adult intact islets, wherein said small islet cell clusters have a diameter of less than 125 μ
m.
-
-
2. A method for forming an implantable device comprising:
-
obtaining intact islets from a pancreas; deriving individual islet cells or small islet cell clusters from said intact islets; and attaching said individual islet cells and small islet cell clusters in a multilayer to a major surface of a substantially planar implantable biomaterial scaffold.
-
-
3. A surface for culturing cells comprising:
-
a substrate comprising a substantially planar top surface, wherein the surface comprises a plurality of divots; wherein each divot comprises an interior bottom surface and an interior sidewall surface, wherein the bottom surface is rounded and wherein each divot is between 100-200 μ
m in diameter (±
20%) and 50-150 μ
m in depth (±
20%);wherein cells are distributed into individual divots. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10)
-
-
11. A method for culturing cells comprising introducing cells to a surface comprising a plurality of divots, wherein each divot is between 100 and 200 μ
- m in diameter (±
20%) and 50 and 150 μ
m in depth (±
20%), and exposing the device to cell culture conditions. - View Dependent Claims (12, 13, 14, 15, 16)
- m in diameter (±
-
17. A method for testing a plurality of cell samples for response to an introduced chemical comprising the steps of (a) culturing cells in a device comprising a plurality of divots, wherein each divot is between 100 and 200 μ
- m in diameter (±
10%) and 50 and 150 μ
m in depth (±
10%), (b) introducing a test chemical to each divot, and (c) analyzing the response of the cells to the test chemical.
- m in diameter (±
-
18. An isolated population of islets wherein at least 80% of all islets are below 90 μ
- m in diameter, the islets have low diffusion barriers relative to native large islets, and wherein the islets have high cell viability relative to native small and large islets, and wherein islet insulin production is characterized by levels that are greater than 10 times more than native isolated islets.
- View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
Specification