FLUID PROCESSING AND VOLUME DETERMINATION SYSTEM

• US 20100233682A1
• Filed: 03/23/2007
• Published: 09/16/2010
• Est. Priority Date: 03/29/2006
• Status: Abandoned Application

• Current Assignee: Koninklijke Philips N.V.
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: De Jong, Michiel, Looijen, Jeroen Pieter
• Application Number:
  US12/294,473
• Publication Number:
  US 20100233682A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01F 31/65 the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01F 22/02 involving measurement of pressure
• CPC Class Codes: Y10T 137/7837 Direct response valves [i.e., check valve type]

Abstract

A fluid processing system is described having at least two chambers. Each of said chambers is separated in a first and a second part by a flexible membrane, the first part, in use, comprising essentially a gas and the second part, in use comprising essentially a non-gaseous fluid, an inlet and/or an outlet means. One or more channels are provided connecting said second parts of said at least two chambers, wherein at least one of said one or more channels includes a pressure sensitive one-way valve. Further, means for exerting pressure on said first part of at least one of said at least two chambers is provided to allow transfer of a sample liquid.

Claims

1. A fluid processing system comprising:

(i) at least two chambers, each of said chambers being separated in a first and a second part by a flexible membrane, said first part comprising essentially a gas and said second part comprising essentially a non-gaseous fluid, an inlet and/or an outlet means,

(ii) one or more channels connecting said second parts of said at least two chambers, wherein at least one of said one or more channels includes a pressure sensitive one-way valve,

(iii) means for exerting pressure on said first part of at least one of said at least two chambers.

2. A fluid processing system according to claim 1 wherein at least one of the said first parts of said at least two chambers is connected to a pressure transducer.

3. A fluid processing system according to claim 1 wherein at least one of the said first parts of said at least two chambers is connected to a temperature sensor.

4. A fluid processing system according to claim 1 wherein said means for exerting pressure is a single pressure means connected via a pressure supply line to each of the at least two chambers, said pressure supply lines comprising one valve for each of said at least two chambers, permitting to connect or disconnect said each of said at least two chambers from said means for exerting pressure.

5. A fluid processing system according to claim 4, wherein said one valve for each of said at least two chambers is a 3/2 valve.

6. A fluid processing system according to claim 1 wherein at least one of said at least two chambers has its said second part directly connected to two or more chambers by one or more channels, wherein at least one of said one or more channels incorporates a pressure sensitive one-way valve.

7. A fluid processing system according to claims 6 wherein said at least one of said at least two chambers is positioned downstream of at least two of said two or more chambers.

8. A fluid processing system according to claim 6 wherein said at least one of said at least two chambers is positioned upstream of at least two of said two or more chambers.

9. A fluid processing system according to claim 5 further comprising an exhaust line connecting the first part of all chambers via said 3/2 valves.

10. A fluid processing system according to claim 9, wherein said exhaust line does not comprises an air reservoir.

11. A fluid processing system according to claim 9 further comprising an air reservoir connected to said exhaust line.

12. A fluid processing system according to claim 6 wherein said at least one of said at least two chambers can selectively connect or disconnect its said second part from/to any of said two or more chambers by selecting positions of a multi-position valve.

13. A fluid processing system according to claim 1 wherein two or more of said at least two chambers differ in size.

14. A method to determine the volume Δ

V of a non-gaseous fluid that has been transferred from one chamber to another chamber of known volume V8 in a fluid processing system according to claim 1, said method comprising the steps of;

(i) measuring the pressure P8 in the first part of the receiving chamber before said transfer,

(ii) measuring the pressure P8′

in the first part of the receiving chamber after said transfer,

(iii) resolving the following equation;

Δ

V=V8(1−

P8/P8′

)

15. A method according to claim 14 comprising instead of step (iii) thereto, the steps of:

(i) measuring the temperature T8 in the first part of the receiving chamber before said transfer,

(ii) measuring the temperature T8′

in the first part of the receiving chamber after said transfer, (iii) resolving the following equation;

Δ

V=V8′

(P8′

T8/T8′

P8−

1).

16. A biosensing device for the analysis of a fluid containing one or more analyte molecules to be detected, said biosensing device comprising:

a fluid processing system comprising;

(i) at least two chambers, each of said chambers being separated in a first and a second part by a flexible membrane, said first part comprising essentially a gas and said second part comprising essentially a non-gaseous fluid, an inlet and/or an outlet means,

(ii) one or more channels connecting said second parts of said at least two chambers, wherein at least one of said one or more channels includes a pressure sensitive one-way valve,

(iii) means for exerting pressure on said first part of at least one of said at least two chambers,

wherein

a) one of the at least two chambers is any of;

a PCR amplification chamber (25), a detection chamber (27), a cell lysing chamber, a purification chamber, a washing chamber, an incubation chamber, a thermal cycling chamber, a cell fragment extraction chamber, or

b) an inlet or an outlet of at least one of the at least two chambers is fluidly connectable to any of;

a PCR amplification chamber (25), a detection chamber (27), a cell lysing chamber, a purification chamber, a washing chamber, an incubation chamber, a thermal cycling chamber, a cell fragment extraction chamber.

17. The biosensing device according to claim 16, the detection chamber including a biosensing solid substrate (30) comprising one or more probes able to specifically bind said one or more analyte molecules.

18. The biosensing device of claim 17, further comprising a detector for analyzing said biosensing substrate after that said sample fluid has contacted said biosensing solid substrate so as to determine the presence of said one or more analyte molecules.

19. A method of processing a sample liquid, said method comprising:

processing the sample liquid using at least two chambers, each of said chambers being separated in a first and a second part by a flexible membrane, said first part comprising essentially a gas and said second part comprising essentially a non-gaseous fluid, an inlet and/or an outlet means, one or more channels connecting said second parts of said at least two chambers, wherein at least one of said one or more channels includes a pressure sensitive one-way valve, the method further comprising exerting pressure on said first part of at least one of said at least two chambers to transfer sample liquid.

20. The method of claim 19, the method being for analysis of a sample liquid containing one or more analyte molecules, at least a step of the method being any of:

PCR amplification, detection, thermal cycling, cell lysing, cell fragment extraction, washing, purification, and incubation.