Tubular nanostructure targeted to cell membrane

• US 20100233781A1
• Filed: 01/30/2009
• Published: 09/16/2010
• Est. Priority Date: 09/15/2008
• Status: Abandoned Application

• Current Assignee: Searete LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Ishikawa, Muriel Y., Myhrvold, Nathan P., Tegreene, Clarence T., Jung, Edward K.Y., Wood, Lowell L. JR., Harlow, Ed, Rivet, Dennis J., Hyde, Roderick A., Sweeney, Elizabeth A., Wood, Victoria Y.H., Bangera, Mahalaxmi Gita, Leuthardt, Eric C.
• Application Number:
  US12/322,366
• Publication Number:
  US 20100233781A1
• Time in Patent Office: Days
• US Class Current: 435/193
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 38/1703 from vertebrates
• CPC Class Codes: A61K 38/1732 Lectins
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: A61K 47/6871 the antibody targeting an enzyme
• CPC Class Codes: A61K 47/6901 Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C01B 21/0648 After-treatment, e.g. grinding, purification transformation of hexagonal into cubic or wurtzitic boron nitride C04B35/5831
• CPC Class Codes: C01B 2202/02 Single-walled nanotubes
• CPC Class Codes: C01B 2202/34 Length
• CPC Class Codes: C01B 2202/36 Diameter
• CPC Class Codes: C01B 32/168 After-treatment
• CPC Class Codes: C01B 32/174 Derivatisation; Solubilisation; Dispersion in solvents
• CPC Class Codes: C01P 2004/13 Nanotubes
• CPC Class Codes: C07K 16/32 against translation products of oncogenes
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
• CPC Class Codes: Y10S 977/743 : having specified tube end structure, e.g. close-ended shell or open-ended tube
• CPC Class Codes: Y10S 977/75 : Single-walled
• CPC Class Codes: Y10S 977/762 : Nanowire or quantum wire, i.e. axially elongated structure having two dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/788 : Of specified organic or carbon-based composition
• CPC Class Codes: Y10S 977/795 : Composed of biological material
• CPC Class Codes: Y10S 977/848 : Tube end modifications, e.g. capping, joining, splicing
• CPC Class Codes: Y10S 977/896 : Chemical synthesis, e.g. chemical bonding or breaking
• CPC Class Codes: Y10S 977/915 : Therapeutic or pharmaceutical composition

Abstract

Devices, compositions, and methods are described which provide a tubular nanostructure targeted to a lipid bilayer membrane. The targeted tubular nanostructure can have a surface region configured to pass through a lipid bilayer membrane of a cell, a hydrophobic surface region flanked by two hydrophilic surface regions configured to form a pore in a lipid bilayer membrane of a cellular organelle, and at least one ligand configured to bind one or more cognates on the lipid bilayer membrane of the cellular organelle. The target cell can be, for example, a tumor cell, an infected cell, or a diseased cell in a subject. The tubular nanostructure can form a pore in the lipid bilayer membrane of the cellular organelle, e.g., mitochondria, which can permit transit or translocation of at least one compound across the membrane and cause cell death of the target cell.

Claims

1-58. -58. (canceled)

59. A composite tubular nanostructure comprising:

two or more nanotubes wherein at least one nanotube includes,

a surface region configured to pass through a lipid bilayer membrane of a cell, and

a hydrophobic surface region flanked by two hydrophilic surface regions configured to form a pore in a lipid bilayer membrane of a cellular organelle.

60. The composite tubular nanostructure of claim 59, further comprising at least one ligand configured to bind one or more cognates on the lipid bilayer membrane of the cellular organelle.

61. The composite tubular nanostructure of claim 60, further comprising at least one second ligand configured to bind one or more cognates on the lipid bilayer membrane of the cell.

62. The composite tubular nanostructure of claim 60, wherein two or more ligands are configured to bind to the one or more cognates on the lipid bilayer membrane of the cellular organelle.

63. The composite tubular nanostructure of claim 59, wherein the composite tubular nanostructure induces cell death.

64. The composite tubular nanostructure of claim 59, wherein the surface region configured to pass through a lipid bilayer membrane of the cell is a hydrophobic surface region or a structured amphiphilic surface region.

65. The composite tubular nanostructure of claim 59, wherein the surface region configured to pass through a lipid bilayer membrane of the cell is configured to interact with a cellular component to produce the at least one tubular nanostructure including the hydrophobic surface region flanked by two hydrophilic surface regions.

66. The composite tubular nanostructure of claim 65, wherein the cellular component is a cytoplasmic component.

67. The composite tubular nanostructure of claim 66, wherein the cytoplasmic component is an esterase which removes a hydrophobic ester from the surface region configured to pass through a lipid bilayer membrane of the cell to produce the at least one tubular nanostructure including the hydrophobic surface region flanked by two hydrophilic surface regions.

68. The composite tubular nanostructure of claim 59, wherein the cell is a neoplastic cell or an infected cell.

69. The composite tubular nanostructure of claim 59, including three or more nanotubes.

70. The composite tubular nanostructure of claim 69, wherein at least one nanotube includes a completely hydrophobic surface region.

71. The composite tubular nanostructure of claim 70, wherein the at least one nanotube including the completely hydrophobic surface region is surrounded by at least six nanotubes including the hydrophobic surface region flanked by two hydrophilic surface regions configured to form the pore in the lipid bilayer membrane.

72. The composite tubular nanostructure of claim 59, wherein at least two of the nanotubes have different diameters.

73. The composite tubular nanostructure of claim 59, wherein at least two of the nanotubes have different lengths.

74. The composite tubular nanostructure of claim 59, wherein the nanotubes are substantially parallel.

75. The composite tubular nanostructure of claim 59, wherein the nanotubes are substantially orthogonal.

76. The composite tubular nanostructure of claim 59, wherein at least one of the two or more nanotubes includes a carbon nanotube, cyclic peptide nanotube, crown ether nanotube, polymer nanotube, polymer/carbon nanotube, DNA nanotube, or inorganic nanotube.

77. The composite tubular nanostructure of claim 59, wherein the hydrophobic surface region includes a single wall carbon nanotube surface region.

78. The composite tubular nanostructure of claim 59, wherein the hydrophilic surface region includes one or more of amines, amides, charged or polar amino acids, alcohols, carboxylic groups, oxides, ester groups, ether groups, ester-ether groups, ketones, aldehydes, or derivatives thereof.

79-101. -101. (canceled)