GENERATION OF PLURIPOTENT STEM CELLS USING RECOMBINANT PROTEINS
First Claim
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1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising:
- contacting the non-pluripotent cell with one or more different exogenous polypeptides, the exogenous polypeptides comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell;
wherein the transcription factor is selected from the group consisting of a Klf polypeptide, an Oct polypeptide, a Myc polypeptide, and a Sox polypeptide.
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Abstract
The present invention provides for methods, compositions, and kits of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell using exogenous transcription factors.
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Citations
38 Claims
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1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising:
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contacting the non-pluripotent cell with one or more different exogenous polypeptides, the exogenous polypeptides comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell; wherein the transcription factor is selected from the group consisting of a Klf polypeptide, an Oct polypeptide, a Myc polypeptide, and a Sox polypeptide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. An isolated polypeptide comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, wherein the transcription factor is selected from the group consisting of:
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an Oct polypeptide, a Klf polypeptide, a Myc polypeptide, and a Sox polypeptide. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification