Substrate Recognition By Differentiable Human Mesenchymal Stem Cells
First Claim
1. A structure for growing isolated differentiable human mesenchymal cells comprising a three-dimensional matrix of electrospun fibers, wherein the matrix of fibers is seeded with the isolated differentiable human mesenchymal cells, such that the isolated differentiable human mesenchymal cells attach to the matrix of fibers and proliferate on the matrix of fibers, and wherein the three-dimensional matrix of fibers forms a supporting scaffold of fibers for promoting differentiation of the isolated differentiable human mesenchymal cells into a mature cell phenotype on the scaffold of fibers.
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Abstract
The invention described herein provides a structure for growing isolated differentiable human mesenchymal cells, which includes a three-dimensional matrix of fibers. The matrix serves as an implantable scaffolding for delivery of differentiable human mesenchymal cells in tissue engineering. The invention further provides compositions that contain the three-dimensional matrix of fibers seeded with isolated differentiable human mesenchymal cells, wherein the matrix forms a supporting scaffold for growing the isolated differentiable human mesenchymal cells, and wherein the differentiable human mesenchymal cells differentiate into a mature cell phenotype. The invention further provides methods of preparing the implantable nanofiber matrix scaffolding seeded with differentiable human mesenchymal cells for use in tissue engineering.
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Citations
20 Claims
- 1. A structure for growing isolated differentiable human mesenchymal cells comprising a three-dimensional matrix of electrospun fibers, wherein the matrix of fibers is seeded with the isolated differentiable human mesenchymal cells, such that the isolated differentiable human mesenchymal cells attach to the matrix of fibers and proliferate on the matrix of fibers, and wherein the three-dimensional matrix of fibers forms a supporting scaffold of fibers for promoting differentiation of the isolated differentiable human mesenchymal cells into a mature cell phenotype on the scaffold of fibers.
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12. A composition for use in tissue engineering comprising:
- isolated differentiable human mesenchymal cells; and
a supporting scaffold for growing the isolated differentiable human mesenchymal cells, the supporting scaffold comprising a three-dimensional matrix of electrospun fibers, wherein the matrix is seeded with the isolated differentiable human mesenchymal cells, wherein the isolated differentiable human mesenchymal cells attach to the matrix of fibers, proliferate on the matrix of fibers, such that the isolated differentiable human mesenchymal cells attach to the matrix of fibers and proliferate on the matrix of fibers, and wherein the supporting scaffold of fibers promotes differentiation of the isolated differentiable human mesenchymal cells into a mature cell phenotype on the scaffold of fibers. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
- isolated differentiable human mesenchymal cells; and
Specification