USES AND METHODS OF MAKING MICROARRAYS OF POLYMERIC BIOMATERIALS

• US 20100234244A1
• Filed: 05/26/2010
• Published: 09/16/2010
• Est. Priority Date: 03/09/2001
• Status: Abandoned Application

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Putnam, David A., Anderson, Daniel G., Langer, Robert S.
• Application Number:
  US12/788,140
• Publication Number:
  US 20100234244A1
• Time in Patent Office: Days
• US Class Current: 506/14
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00

Abstract

A microarray of polymeric biomaterials is provided. Specifically, a microarray of polymeric biomaterials that comprises a base with a cytophobic surface, and a plurality of discrete polymeric biomaterial elements bound to the cytophobic surface, is provided. Preferably said polymeric biomaterials comprise a synthetic polymer. Said polymeric biomaterials may also comprise other compounds covalently or non-covalently attached to said synthetic polymer. Methods of preparing the microarray of polymeric biomaterials of the present invention and uses of the microarray of polymeric biomaterials of the present invention are also provided.

Claims

1. An microarray, comprising:

a plurality of synthetic polymeric biomaterial elements that are bound to a cytophobic surface, wherein the polymeric biomaterial elements are deposited at discrete locations on the surface, and wherein the synthetic polymeric biomaterial elements are not proteins or polynucleotides.

2. The microarray of claim 1, wherein the cytophobic surface comprises a hydrogel.

3. The microarray of claim 2, wherein the hydrogel comprises a polymer selected from the group consisting of homopolymers of methacrylic acid esters, homopolymers of alkylene oxides, homopolymers of alkylene glycols, copolymers thereof, and mixtures thereof.

4. The microarray of claim 2, wherein the hydrogel comprises a polymer selected from the group consisting of poly(methyl methacrylate), poly(isobutyl methacrylate), poly(pentyl methacrylate), poly(2-hydroxy-ethyl methacrylate), copolymers thereof, and mixtures thereof.

5. The microarray of claim 2, wherein the hydrogel comprises a polymer selected from the group consisting of poly(ethylene oxide), poly(propylene 1,2-glycol), poly(propylene 1,3-glycol), copolymers thereof, and mixtures thereof.

6. The microarray of claim 1, wherein the polymeric biomaterial elements are not covalently bound to the cytophobic surface.

7. The microarray of claim 6, wherein the polymeric biomaterial elements are bound to the cytophobic surface via a non-covalent interaction selected from the group consisting of chemical adsorption, hydrogen bonding, surface interpenetration, ionic bonding, van der Waals forces, hydrophobic interactions, magnetic interactions, dipole-dipole interactions, and combinations thereof.

8. The microarray of claim 1, wherein the polymeric biomaterial elements are not monolayers.

9. The microarray of claim 1, wherein each of the polymeric biomaterial elements comprises at least one polymer selected from the group consisting of synthetic polymers, adducts thereof, and mixtures thereof.

10. The microarray of claim 9, wherein the synthetic polymers are selected from the group consisting of polyamides, polyphosphazenes, polypropylfumarates, synthetic poly(amino acids), polyethers, polyacetals, polycyanoacrylates, polyurethanes, polycarbonates, polyanhydrides, poly(ortho esters), polyhydroxyacids, polyesters, polyacrylates, ethylene-vinyl acetate polymers, cellulose acetates, polystyrenes, poly(vinyl chloride), poly(vinyl fluoride), poly(vinyl imidazole), poly(vinyl alcohol), and chlorosulphonated polyolefins.

11. The microarray of claim 9, wherein at least one of the polymeric biomaterial elements further comprises a compound selected from the group consisting of drugs, growth factors, combinatorial compounds, proteins, polysaccharides, polynucleotides, lipids, adducts thereof, and mixtures thereof.

12. The microarray of claim 11, wherein the compound is covalently bound to the synthetic polymer component or components of the polymeric biomaterial.

13. The microarray of claim 11, wherein the compound is non-covalently bound to the synthetic polymer component or components of the polymeric biomaterial.

14. The microarray of claim 1, wherein the polymeric biomaterial elements are between 10 and 1000 μ

m in diameter.

15. The microarray of claim 1, wherein the polymeric biomaterial elements are between 50 and 500 μ

m in diameter.

16. The microarray of claim 1, wherein:

the microarray is a rectangular microarray; and

the polymeric biomaterial elements are disposed at between 100 and 1200 μ

m intervals on the cytophobic surface.

17. The microarray of claim 1, wherein:

the microarray is a rectangular microarray; and

the polymeric biomaterial elements are disposed at between 300 and 500 μ

m intervals on the cytophobic surface.

18. The microarray of claim 1, wherein the polymeric biomaterial elements are present at a density on the cytophobic surface that ranges from 1 to 1,000 polymeric biomaterial elements per cm².

19. The microarray of claim 1, wherein the polymeric biomaterial elements are present at a density on the cytophobic surface that ranges from 10 to 100 polymeric biomaterial elements per cm².

20. The microarray of claim 1, further comprising a cell.

21. The method of claim 20, wherein the cell is selected from the group consisting of mammalian cells, bacterial cells, yeast cells, and plant cells.

22. The method of claim 20, wherein the cell is selected from the group of mammalian cells consisting of chondrocytes, fibroblasts, connective tissue cells, epithelial cells, endothelial cells, cancer cells, hepatocytes, islet cells, smooth muscle cells, skeletal muscle cells, heart muscle cells, kidney cells, intestinal cells, organ cells, lymphocytes, blood vessel cells, stem cells, human embryonic stem cells, and mesenchymal stem cells.

23. The microarray of claim 1, wherein at least one of the polymeric biomaterial elements comprises a compound.

24. The microarray of claim 23, wherein the compound is a drug approved for human use by the U.S. Food and Drug Administration.

25. The microarray of claim 23, wherein the compound belong to a synthetic combinatorial library of compounds.

26. The microarray of claim 23, wherein the compound are selected from the group consisting of proteins, polysaccharides, polynucleotides, lipids, adducts thereof, and mixtures thereof.

27. The microarray of claim 23, wherein the compound is a drugs approved for veterinary use by the U.S. Food and Drug Administration.

28. The microarray of claim 1, further comprising a cell and a compound.

29. The microarray of claim 1, further comprising a human cell.

30. The microarray of claim 1, further comprising a cancer cell.