MICROFLUIDIC SYSTEMS AND METHODS
First Claim
10. A microfluidic system for assaying a plurality of cells, the microfluidic system comprising:
- a substrate; and
a plurality of microfluidic channels arranged in the substrate, each of said plurality of microfluidic channels comprising;
a source channel at an end of said microfluidic channel and a sink channel at an opposing end, said source channel having an inlet and an outlet;
a cell chamber disposed within said microfluidic channel between said source channel and said sink channel;
a cell inlet in fluid communication with said cell chamber; and
a fluidic resistance disposed between said cell chamber and one of said sink channel and said source channel.
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Accused Products
Abstract
Microfluidic systems and methods. A microfluidic device for in vitro fertilization comprises a substrate and a plurality of microchannels disposed in the substrate, including an inlet of at least two of the plurality of microchannels arranged on the substrate to align with a fluid-handling device. Another microfluidic system for assaying a plurality of cells comprises a substrate and a plurality of microfluidic channels comprising a source channel, a sink channel, and a cell chamber. An insert for a microfluidic system comprises a substrate configured to be inserted into a dish and a plurality of microscale wells disposed in the substrate. A microfluidic channel comprises a substrate and at least one microchannel having an open inlet, an open outlet, a channel, and an opening in the substrate disposed over a portion of the channel. A device for providing an amount of fluid for a fluidic system comprises a main reservoir, an aspiration well, tubing coupling the reservoirs, and a seal closing the main reservoir. Air tubing having a hydrophobic end extends into the at least one aspiration well.
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Citations
42 Claims
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10. A microfluidic system for assaying a plurality of cells, the microfluidic system comprising:
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a substrate; and a plurality of microfluidic channels arranged in the substrate, each of said plurality of microfluidic channels comprising; a source channel at an end of said microfluidic channel and a sink channel at an opposing end, said source channel having an inlet and an outlet; a cell chamber disposed within said microfluidic channel between said source channel and said sink channel; a cell inlet in fluid communication with said cell chamber; and a fluidic resistance disposed between said cell chamber and one of said sink channel and said source channel. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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22-1. The microfluidic system of claim 21, further comprising:
at least one channel disposed in said substrate and fluidly coupling at least two of said plurality of microscale wells.
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29. A method of fabricating a microfluidic system comprising:
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providing a substrate; fabricating, using soft lithography, an array of microscale wells in said substrate, wherein fabrication of each of said microscale wells is controlled to have a predetermined size, depth, and distance therebetween; inserting said substrate into a dish; and attaching said substrate to the dish.
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30. A microfluidic channel comprising:
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a substrate; at least one microchannel disposed in said substrate, wherein each of said at least one microchannel comprises; an open inlet at one end; an open outlet at an opposing end; a channel within said substrate and disposed between said inlet and said outlet; and an opening in said substrate disposed over a portion of said channel to expose an open portion of said channel. - View Dependent Claims (31, 32, 33, 34, 35)
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36. For a fluidic system, a device for providing an amount of fluid comprising:
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a first reservoir providing a main reservoir; at least one second reservoir separate from said first reservoir, said at least one second reservoir providing an aspiration well; a siphon tubing coupling said first reservoir and said at least one second reservoir; a seal closing said first reservoir; an air tubing extending through a portion of said seal, said air tubing being coupled to said at least one second reservoir and having an end extending into said at least one second reservoir; and wherein the end of said air tubing is hydrophobic. - View Dependent Claims (37, 38, 39, 40, 41)
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42. A method of dispensing a fluid comprising:
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providing a first reservoir providing a main reservoir, at least one second reservoir separate from said first reservoir and providing an aspiration well, a siphon tubing coupling said first reservoir and said at least one second reservoir, a seal closing said first reservoir, and an air tubing extending through a portion of said seal, wherein said air tubing is coupled to said at least one second reservoir and has an end extending into said at least one second, reservoir, and wherein the end of said air tubing is hydrophobic; filling a portion of the first reservoir with a liquid; closing the seal to close the first reservoir, wherein an opposite end of the air tubing is disposed through an opening of the seal; and inducing a negative pressure in the at least one second reservoir, wherein the liquid is dispensed from said first reservoir to said at least one second reservoir via the siphon tubing until the dispensed liquid reaches the hydrophobic end of the air tubing.
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Specification