Two Dimensional Imaging of Reacted Areas On a Reagent
First Claim
1. A system for reading an assay, the assay having a dry reagent that defines a test area and a background area;
- the assay being used as a test for a reaction between a sample and the reagent, the test area of the reagent being in contact with the sample, the system comprising;
a camera that simultaneously captures a first two-dimensional image of the test area and the background area; and
a processor that determines, from the first two-dimensional image, a first color response from the background area and a second color response from the test area, calibrates the second color response according to the first color response, and generates a result of the test according to the second color response.
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Accused Products
Abstract
A system for reading an assay includes a camera and a processor. The assay is used as a test for the presence or absence of a reaction between a sample and a reagent. The assay is defined by a test area and a background area. The test area is, preferably, substantially circular with a diameter between about 0.1 mm and about 5 mm. The camera simultaneously captures a two-dimensional image of the test area and the background area. The processor determines a first color response from the background area and a second color response from the test area. The processor then calibrates the second color response according to the first color response and generates a result of the test according to the second color response. The system may also include a uniform field illuminator that provides a substantially uniform level of illumination across the assay.
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Citations
41 Claims
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1. A system for reading an assay, the assay having a dry reagent that defines a test area and a background area;
- the assay being used as a test for a reaction between a sample and the reagent, the test area of the reagent being in contact with the sample, the system comprising;
a camera that simultaneously captures a first two-dimensional image of the test area and the background area; and a processor that determines, from the first two-dimensional image, a first color response from the background area and a second color response from the test area, calibrates the second color response according to the first color response, and generates a result of the test according to the second color response. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- the assay being used as a test for a reaction between a sample and the reagent, the test area of the reagent being in contact with the sample, the system comprising;
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21. A system for reading an assay;
- the assay having a dry reagent that defines a plurality of test areas separated by a background area;
the assay being used as a test for a reaction between a sample and the dry reagent, system comprising;a camera that captures a plurality of two-dimensional images, wherein at least a first two-dimensional image of the plurality of two-dimensional images includes the background area and a first subset of the plurality of test areas; and a processor that determines, from the plurality of two-dimensional images, a first color response of the background area and a plurality of respective second color responses corresponding to the first subset of the plurality of test areas, calibrates the plurality of respective second color responses according to the first color response, and generates a first plurality of respective results according to the plurality of respective second color responses. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
- the assay having a dry reagent that defines a plurality of test areas separated by a background area;
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37. A computer readable medium for reading an assay, the assay having a dry reagent that defines a test area and a background area;
- the assay being used as a test for a reaction between a sample and the reagent, the test area of the reagent being in contact with the sample, the computer-readable medium having computer instructions stored thereon that when executed perform a method comprising;
receiving a plurality of two-dimensional images that include the test area and the background area; determining, from the plurality of two-dimensional images, a first color response from the background area; determining, from plurality of two-dimensional images, a second color response from the test area; calibrating the second color response according to the first color response; and generating a result of the test according to the second color response. - View Dependent Claims (38, 39, 40, 41)
- the assay being used as a test for a reaction between a sample and the reagent, the test area of the reagent being in contact with the sample, the computer-readable medium having computer instructions stored thereon that when executed perform a method comprising;
Specification