Methods and Products For In Vivo Enzyme Profiling
First Claim
1. A method comprisingadministering to a subject a pro-diagnostic reagent, wherein the pro-diagnostic reagent comprises a modular structure having a carrier domain linked to a signature producing domain, wherein the signature producing domain is capable of producing a signature molecule in the subject;
- identifying a biological sample for detection of the signature molecule, wherein the biological sample is at a site remote from the production of the signature molecule; and
,subjecting the biological sample to an analysis method in order to detect the presence of the signature molecule, wherein the presence of the signature molecule in the biological sample is indicative of a biological predictor molecule within the subject.
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Accused Products
Abstract
The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
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Citations
82 Claims
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1. A method comprising
administering to a subject a pro-diagnostic reagent, wherein the pro-diagnostic reagent comprises a modular structure having a carrier domain linked to a signature producing domain, wherein the signature producing domain is capable of producing a signature molecule in the subject; -
identifying a biological sample for detection of the signature molecule, wherein the biological sample is at a site remote from the production of the signature molecule; and
,subjecting the biological sample to an analysis method in order to detect the presence of the signature molecule, wherein the presence of the signature molecule in the biological sample is indicative of a biological predictor molecule within the subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 16, 17, 24, 32, 39, 40, 41, 42, 43)
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10-15. -15. (canceled)
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18-23. -23. (canceled)
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25-31. -31. (canceled)
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33-38. -38. (canceled)
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44-50. -50. (canceled)
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51. A method comprising
collecting a urine sample from a subject suspected of having a disorder or condition, wherein the subject has been administered a pro-diagnostic reagent, the pro-diagnostic reagent comprising a carrier domain linked to an enzyme susceptible domain which is linked to a signature molecule; - and,
subjecting the urine sample to a multiplex analysis method in order to detect the presence of the signature molecule, and wherein the presence or absence of the signature molecule in the biological sample is indicative of the disorder or condition within the subject.
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52-80. -80. (canceled)
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81. A reagent comprising
a carrier domain having a plurality of enzyme susceptible domains linked to the carrier domain wherein each enzyme susceptible domain is linked to a non-fluorescent signature molecule.
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82-88. -88. (canceled)
Specification