METHODS AND POLYNUCLEOTIDES FOR IMPROVING PLANTS

• US 20100242134A1
• Filed: 07/25/2008
• Published: 09/23/2010
• Est. Priority Date: 07/26/2007
• Status: Abandoned Application

• Current Assignee: Catherine Jane Bryant, Jonathan Robert Phiillips, Kieran Michael Elborough, Sathish Puthigae, Shivendra Bajaj
• Original Assignee: Catherine Jane Bryant, Jonathan Robert Phiillips, Kieran Michael Elborough, Sathish Puthigae, Shivendra Bajaj
• Inventors: Elborough, Kieran Michael, Phiillips, Jonathan Robert, Bajaj, Shivendra, Bryant, Catherine Jane, Puthigae, Sathish
• Application Number:
  US12/670,643
• Publication Number:
  US 20100242134A1
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: C12N 15/8267 Seed dormancy, germination or sprouting
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The invention provides methods for producing a plant with altered tillering time, the methods comprising transformation of a plant with a genetic construct including a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID NO: 1 or a variant or fragment thereof. The method also provides isolated polypeptides, polynucleotides, constructs and vectors useful for producing a plant with altered tillering time. The method also provides plant cell and plants transformed to contain and express the polypeptides, polynucleotides and constructs. The invention also provides plants produced by methods of the invention.

Claims

1-37. -37. (canceled)

38. A method for producing a plant with altered tillering time, the method comprising transformation of a plant cell or plant with a:

a) a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID NO;

1 or a variant of the polypeptide;

b) a polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a);

or

c) a polynucleotide comprising a compliment, of at least 15 nucleotides in length, of the polynucleotide of a).

39. The method of claim 38 wherein the variant in a) is capable of modulating tillering time in a plant.

40. The method of claim 38 in which the variant has at least 70% sequence identity to a polypeptide with the amino acid sequence of SEQ ID NO:

1.

41. The method of claim 38 in which the variant is derived from a plant species and comprises the amino acid sequence of SEQ ID NO:

25.

42. The method of claim 38 in which the variant is derived from a monocotyledonous species and comprises the amino acid sequence of at least one of SEQ ID NO:

10 and SEQ ID NO;

11.

43. The method of claim 38 in which the variant is derived from a dicotyledonous species and comprises the amino acid sequence of at least one of SEQ ID NO:

23 and SEQ ID NO;

24.

44. The method of claim 38 in which the variant comprises an amino acid sequence selected from any one of SEQ ID NO:

2-9.

45. The method of claim 38 in which the polynucleotide of a) encodes a polypeptide with the amino acid sequence of SEQ ID NO:

1.

46. The method of claim 38 in which the polynucleotide of a) comprises a sequence with at least 70% sequence identity to SEQ ID NO:

12.

47. The method of claim 38 in which the polynucleotide of a) comprises the sequence of any one of SEQ ID NO:

14 to 21.

48. The method of claim 38 in which the polynucleotide of a) comprises the coding sequence of any one of SEQ ID NO:

14 to 21.

49. The method of claim 38 in which the polynucleotide of a) comprises the sequence of SEQ ID NO:

12.

50. The method of claim 38 in which the polynucleotide of a) comprises the coding sequence of SEQ ID NO:

12.

51. The method of claim 38 in which the plant with altered tillering time is early tillering relative to a suitable control plant.

52. The method of claim 38 in which the plant with altered tillering time is late tillering relative to a suitable control plant.

53. A plant cell or plant produced by a method of claim 38.

54. An isolated polynucleotide encoding a polypeptide with at least 70% identity to the amino acid sequence selected of SEQ ID NO:

1.

55. The isolated polynucleotide of claim 54 wherein the polypeptide is capable of modulating tillering time in a plant.

56. The isolated polynucleotide of claim 54 comprising the sequence of SEQ ID NO:

12.

57. The isolated polynucleotide sequence of claim 54 comprising the full-length coding sequence of SEQ ID NO:

12.

58. The isolated polynucleotide sequence of claim 54 comprising the sequence of SEQ ID NO:

13.

59. The isolated polynucleotide sequence of claim 54 comprising a sequence with at least 70% identity to SEQ ID NO:

12.

60. The isolated polynucleotide sequence of claim 54 comprising the sequence of SEQ ID NO:

12.

64. A genetic construct which comprises the polynucleotide of claim 54.

65. A genetic construct including a polynucleotide consisting of at least one of:

a) a fragment, of at least 15 nucleotides in length, of a polynucleotide of claim 54;

b) a complement, of at least 15 nucleotides in length, of the polynucleotide of claim 54;

or

c) a sequence, of at least 15 nucleotides in length, capable of hybridising to the polynucleotide of claim 54.

66. A host cell, plant cell, or plant genetically modified to express a polynucleotide of claim 54.

68. A host cell, plant cell, or plant comprising a genetic construct including a polynucleotide consisting of at least one of:

a) a fragment, of at least 15 nucleotides in length, of a polynucleotide of claim 54;

b) a complement, of at least 15 nucleotides in length, of the polynucleotide of claim 54;

or

c) a sequence, of at least 15 nucleotides in length, capable of hybridising to the polynucleotide of claim 54.

69. A method for selecting a plant with altered tillering time relative to suitable control plant, the method comprising testing of a plant for altered expression of a polynucleotide of claim 54.

61. An isolated polypeptide having at least 70% sequence identity to the amino acid sequence of SEQ ID NO:

1.

62. The isolated polypeptide of claim 61, wherein the polypeptide is capable of modulating tillering time in a plant.

63. The isolated polypeptide of claim 61 comprising the amino acid sequence of SEQ ID NO:

1.

67. A host cell, plant cell, or plant genetically modified to express a polypeptide of claim 61.

70. A method for selecting a plant with altered tillering time relative to suitable control plant, the method comprising testing of a plant for altered expression of a polypeptide of claim 61.

71. An antibody raised against a polypeptide of claim 61.