Biosensor, thin film electrode forming method, quantification apparatus, and quantification method

• US 20100243443A1
• Filed: 06/09/2010
• Published: 09/30/2010
• Est. Priority Date: 11/15/1999
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Tokunaga, Hiroyuki, Tokuno, Yoshinobu, Yamanishi, Eriko, Fujiwara, Masaki, Miyazaki, Shoji
• Granted Patent: US 8,142,629 B2
• Time in Patent Office: Days
• US Class Current: 204/403.140
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/48771 Coding of information, e.g. calibration data, lot number
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1.

The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.

Claims

1-44. -44. (canceled)

45. A biosensor comprising:

a first support;

a second support which is formed at a position opposite to the first support;

an electrode part comprising at least one of a first electrode and a second electrode which are formed on the first and the second support, respectively;

a specimen supply path for introducing a sample liquid to the electrode part; and

a reagent layer which is formed in the specimen supply path, wherein

the respective electrodes of the electrode part are separated from each other by 0.005 to 0.3 mm.

46. The biosensor as defined in claim 45, wherein the specimen supply path and the reagent layer are provided between the first support and the second support, and a spacer is provided with an approximate rectangular cutout part for forming the specimen supply path.

47. The biosensor as defined in claim 45, wherein an electrical conductive layer is formed on the first or the second support, and the respective electrodes are separated from each other by a slit that is formed by a laser.

48. The biosensor as defined in claim 47, wherein the thickness of the electrical conductive layer is less than 100 nm.

49. The biosensor as defined in claim 48, wherein the thickness of the electrical conductive layer is 3 to 50 nm.

50. The biosensor as defined in any of claims 45 to 49, wherein the reagent layer includes an enzyme.

51. The biosensor as defined in any of claims 45 to 49, wherein the reagent layer includes an electron transfer agent.

52. The biosensor as defined in any of claims 45 to 49, wherein the reagent layer includes a hydrophilic polymer.