Synthetic Surfaces for Differentiating Stem Cells into Cardiomyocytes
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Abstract
Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence.
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Citations
37 Claims
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1-25. -25. (canceled)
- 26. A method of differentiating a pluripotent stem cell into a cardiomyocyte comprising culturing the pluripotent stem on a surface comprising a swellable (meth)acrylate layer conjugated to an RGD peptide and contacting the pluripotent stem cells with a media comprising one or more growth factors.
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