Method of measuring quantity of substrate

• US 20100252454A1
• Filed: 06/10/2010
• Published: 10/07/2010
• Est. Priority Date: 11/30/2000
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Company Limited (PHC Holdings Corporation)
• Inventors: Tokunaga, Hiroyuki, Tokuno, Yoshinobu, Miyazaki, Shoji, Matsumoto, Kazuhiro, Sawada, Yasuhiko
• Granted Patent: US 8,668,820 B2
• Time in Patent Office: Days
• US Class Current: 205/792
• CPC Class Codes: A61B 2562/0295 Strip shaped analyte sensors for apparatus classified in A61B5/145 or A61B5/157 disposable multilayered or laminated electrodes G01N27/307; details of handling chemical test elements G01N33/4875
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/48771 Coding of information, e.g. calibration data, lot number
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A method of measuring a quantity of a substrate contained in sample liquid is provided. This method can reduce measurement errors caused by a biosensor. The biosensor includes at least a pair of electrodes on an insulating board and is inserted into a measuring device which includes a supporting section for supporting detachably the biosensor, plural connecting terminals to be coupled to the respective electrodes, and a driving power supply which applies a voltage to the respective electrodes via the connecting terminals. One of the electrodes of the biosensor is connected to the first and second connecting terminals of the measuring device only when the biosensor is inserted into the measuring device in a given direction, and has a structure such that the electrode becomes conductive between the first and second connecting terminals due to a voltage application by the driving power supply.

Claims

1-33. -33. (canceled)

34. A method of measuring a quantity of a substrate, comprising the steps of:

i) providing a biosensor which includes;

an insulating layer;

an electrode section formed on at least a part of the insulating layer, the electrode section including a counter electrode and a measuring electrode; and

a reagent layer for reacting with a sample liquid supplied to the electrode section;

ii) providing a measuring device which includes;

a supporting section for detachably supporting the biosensor; and

a connecting terminal and a driving power supply for applying a voltage to the electrodes of the electrode section;

iii) applying a first voltage from the measuring device to the electrode section during a first period;

iv) removing the first voltage during a standby period after the first period; and

v) measuring a quantity of a substrate in the sample liquid by detecting an electric current produced by applying a second voltage to the electrode section during a second period, after the standby period, the first voltage being greater than the second voltage.

35. The method of claim 34, wherein the standby period is not shorter than 2 seconds and is shorter than 10 seconds.

36. The method of claim 35,

wherein the first voltage is not less than 0.1V and not more than 0.8V, and

wherein the first period is not shorter than 2 seconds and not longer than 10 seconds.

37. The method of claim 36,

wherein the second voltage is not less than 0.05V and not more than 0.6V, and

wherein the second period is not shorter than 2 seconds and not longer than 10 seconds.