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AMINO ACID SUBSTITUTED MOLECULES

  • US 20100254943A1
  • Filed: 11/02/2009
  • Published: 10/07/2010
  • Est. Priority Date: 05/02/2006
  • Status: Active Grant
First Claim
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1. A method for producing a modified target polypeptide, comprising:

  • (a) providing a host cell, the host cell comprising a vector having a polynucleotide encoding the target polypeptide,(b) site-specifically incorporating one or more non-natural amino acid codons into the polynucleotide, wherein at least one non-natural amino acid codon corresponds to the first position of the amino terminus of the target polypeptide,(c) growing the host cell under conditions such that the host cell expresses the modified target polypeptide, wherein the modified target polypeptide comprises a non-natural amino acid residue at the first position of the amino terminus, and wherein the non-natural amino acid residue comprises an azide, alkyne, vinyl, or aryl halide group, thereby producing a modified target polypeptide.

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