Methods For Making And Imaging Arrays
First Claim
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1. A method of making and imaging arrays, comprising:
- a) providing i) a plurality of different biomolecules, and ii) a plurality of beads, said beads comprising a binding agent capable of binding to said biomolecules;
b) capturing a first biomolecule on a first bead with said binding agent so as to create a first reacted bead;
c) capturing a second biomolecule on a second bead with said binding agent so as to create a second reacted bead;
d) creating an array with said first and second reacted beads;
e) imaging said array; and
f) subjecting said array to mass spec analysis.
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Abstract
Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).
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Citations
35 Claims
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1. A method of making and imaging arrays, comprising:
- a) providing i) a plurality of different biomolecules, and ii) a plurality of beads, said beads comprising a binding agent capable of binding to said biomolecules;
b) capturing a first biomolecule on a first bead with said binding agent so as to create a first reacted bead;
c) capturing a second biomolecule on a second bead with said binding agent so as to create a second reacted bead;
d) creating an array with said first and second reacted beads;
e) imaging said array; and
f) subjecting said array to mass spec analysis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
- a) providing i) a plurality of different biomolecules, and ii) a plurality of beads, said beads comprising a binding agent capable of binding to said biomolecules;
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12. A method of making and imaging arrays, comprising:
- a) providing i) a plurality of different proteins, each protein comprising a shared epitope, and ii) a plurality of beads, said beads comprising an antibody specific to said epitope;
b) capturing a first protein on a first bead with said antibody so as to create a first reacted bead;
c) capturing a second protein on a second bead with said antibody so as to create a second reacted bead;
d) creating an array with said first and second reacted beads;
e) imaging said array; and
f) subjecting said array to mass spec analysis. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
- a) providing i) a plurality of different proteins, each protein comprising a shared epitope, and ii) a plurality of beads, said beads comprising an antibody specific to said epitope;
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25. An array comprising first and second beads, said first bead comprising a first mass tag and a first biomolecule, said second bead comprising a second mass tag and a second biomolecule, wherein said first and second biomolecules are different and said first and second mass tags are of different mass.
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26. The array of claim 26, wherein said first and second mass tags are peptide mass tags.
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27. A method of making an array, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first biomolecule, said second bead comprising a second mass tag and a second biomolecule, wherein said first and second biomolecules and said first and second mass tags are different;
- b) making an array with said mixture.
- View Dependent Claims (28)
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29. A method of analyzing an array, comprising a) providing a mixture comprising first and second beads, said first bead comprising a first mass tag and a first biomolecule, said second bead comprising a second mass tag and a second biomolecule, wherein said first and second biomolecules and said first and second mass tags are different;
- b) making an array with said mixture; and
c) subjecting said array to mass spec analysis. - View Dependent Claims (30, 31, 32, 33, 34, 35)
- b) making an array with said mixture; and
Specification