Enrichment of Cells
First Claim
1. A method of isolating and enriching mesenchymal stem cells (MSCs) comprising:
- (a) treating a tissue sample comprising cells and extracellular matrix with an amount of a collagenase that is sufficient to free MSCs from the extracellular matrix in a liquid medium; and
(b) isolating a fraction of the medium containing MSCs.
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Accused Products
Abstract
The present invention relates to methods of isolating and enriching mesenchymal stem cells (MSCs) comprising treating a tissue sample comprising cells and extracellular matrix with an amount of a collagenase that is sufficient to free MSCs from the extracellular matrix in a liquid medium and then isolating a fraction of the medium containing MSCs. The isolated MSCs can be isolated in surprisingly large numbers such that the fraction can have immediate use in a number of clinical contexts. This represents an advance of prior art techniques that either require the pooling of large volumes of sample tissues from different sources or require MSCs to be expanded in culture. The invention therefore further concerns the clinical use of cells isolated according to the methods of the first aspect of the invention.
30 Citations
30 Claims
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1. A method of isolating and enriching mesenchymal stem cells (MSCs) comprising:
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(a) treating a tissue sample comprising cells and extracellular matrix with an amount of a collagenase that is sufficient to free MSCs from the extracellular matrix in a liquid medium; and (b) isolating a fraction of the medium containing MSCs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A kit for enriching mesenchymal stem cells (MSC) comprising a collagenase.
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22. A method of performing cell therapy on a subject in need of such therapy comprising:
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(a) obtaining a tissue sample comprising MSCs and extracellular matrix from said subject; (b) treating said tissue sample with an amount of a collagenase that is sufficient to free MSCs from the extracellular matrix in a liquid medium; (c) isolating a fraction of the medium containing MSCs; and (d) re-introducing said fraction into said subject at a body site that permits said subject to benefit from said cell therapy. - View Dependent Claims (24, 25, 26, 27, 28, 29)
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23. A method of performing cell therapy on a subject in need of such therapy comprising:
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(a) obtaining a tissue sample comprising MSCs and extracellular matrix from a cadaver or donor; (b) treating said tissue sample with an amount of a collagenase that is sufficient to free MSCs from the extracellular matrix in a liquid medium; (c) isolating a fraction of the medium containing MSCs; and (d) introducing said fraction into said subject at a body site that permits said subject to benefit from said cell therapy.
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30. (canceled)
Specification