ENZYME ELECTRODE

• US 20100261072A1
• Filed: 09/18/2008
• Published: 10/14/2010
• Est. Priority Date: 09/18/2007
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: Ultizyme International Ltd.
• Inventors: Tsugawa, Wakako, Sode, Koji
• Granted Patent: US 9,617,576 B2
• Time in Patent Office: Days
• US Class Current: 429/401
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875

Abstract

The present invention provides an enzyme electrode composed of a carbon particle on which glucose dehydrogenase (GDH) with flavine adenine dinucleotide (FAD) as a coenzyme is supported and an electrode layer contacting the carbon particle, wherein the carbon particle and/or the electrode layer are/is composed of the carbon particles with a particle diameter of not more than 100 nm and a specific surface area of at least 200 m²/g.

Claims

1. An enzyme electrode comprising carbon particles carrying glucose dehydrogenase (GDH) with flavine adenine dinucleotide (FAD) as a coenzyme;

and an electrode layer contacting said carbon particles.

2. The enzyme electrode according to claim 1, wherein the glucose dehydrogenase (GDH) is an oxidoreductase catalytic subunit or a complex of an oxidoreductase catalytic subunit and an electron transfer subunit.

3. The enzyme electrode according to claim 1, wherein the electrode layer is composed of metal.

4. The enzyme electrode according to claim 3, wherein the electrode layer is composed of metal wire.

5. A glucose sensor using the enzyme electrode according to claim 1.

9. The enzyme electrode according to claim 1, wherein the carbon particles and/or the electrode layer are/is composed of carbon particles with a particle diameter of not more than 100 nm and a specific surface area of at least 200 m²/g.

12. The enzyme electrode according to claim 1, wherein the carbon particle is VULCAN™

, Ketchen black™

, or Lion Paste™

.

16. An enzyme fuel cell using the enzyme electrode according to claim 1.

17. The enzyme fuel cell according to claim 16, wherein the enzyme electrode is used as the anode.

6. An ink material to be used for an enzyme electrode comprising glucose dehydrogenase (GDH) with flavine adenine dinucleotide (FAD) as a coenzyme;

and carbon particles.

7. The ink material according to claim 6, wherein the glucose dehydrogenase (GDH) is an oxidoreductase catalytic subunit or a complex of an oxidoreductase catalytic subunit and an electron transfer subunit.

8. A method for producing an enzyme electrode, comprising coating a surface of an electrode layer with the ink material according to claim 6 and then drying.

10. The ink material according to claim 6, wherein the carbon particles are composed of carbon particles with a particle diameter of not more than 100 nm and a specific surface area of at least 200 m²/g.

11. The ink material according to claim 6, wherein the carbon particle is VULCAN™

, Ketchen black™

, or Lion Paste™

.

13. The method for producing an enzyme electrode according to claim 8, further comprising adding a solid polyelectrolyte dropwise and then drying, after the step of coating a surface of an electrode layer with the ink material or after the step of drying.

14. The method for producing an enzyme electrode according to claim 8, wherein the ink material further comprises the solid polyelectrolyte.

15. The method for producing an enzyme electrode according to claim 8, wherein the solid polyelectrolyte is Naflon™

.