PRIMERS AND PROBES FOR DETECTING HEPATITIS C VIRUS

• US 20100261154A1
• Filed: 12/29/2009
• Published: 10/14/2010
• Est. Priority Date: 12/31/2008
• Status: Abandoned Application

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Esping, Claudia, Schneider, George
• Application Number:
  US12/648,311
• Publication Number:
  US 20100261154A1
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/707 non-A, non-B Hepatitis, excluding hepatitis D

Abstract

The present invention relates to primers, probes, primer sets, primer and probe sets, methods and kits for detecting Hepatitis C virus in a test sample.

Claims

1. A primer for amplifying Hepatitis C virus in a test sample, wherein the primer has a sequence selected from the group consisting of:

SEQ ID NO;

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, and complements thereof.

2. A probe for detecting Hepatitis C virus in a test sample, wherein the probe has a sequence selected from the group consisting of:

SEQ ID NO;

7, SEQ ID NO;

8, and complements thereof.

3. A primer set for amplifying Hepatitis C virus in a test sample, the primer set comprising:

(a) at least one forward primer having a sequence selected from the group consisting of;

SEQ ID NO;

1, SEQ ID NO;

2, complements thereof, and any combinations thereof; and

(b) at least one reverse primer having a sequence selected from the group consisting of;

SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, complements thereof, and any combinations thereof.

4. A primer and probe set for detecting Hepatitis C virus in a test sample, comprising:

(a) two forward primers having a sequence of;

SEQ ID NO;

1 and SEQ ID NO;

2, or complements thereof, and four reverse primers having a sequence of;

SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, and SEQ ID NO;

6, or complements thereof; and

(b) two probes having a sequence of;

SEQ ID NO;

7 and SEQ ID NO;

8, or complements thereof.

5. A method for detecting Hepatitis C virus in a test sample, the method comprising the steps of:

(a) contacting a test sample with at least one forward primer having a sequence selected from the group consisting of SEQ ID NO;

1 and SEQ ID NO;

2, or complements thereof and at least one reverse primer having a sequence selected from the group consisting of;

SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, and SEQ ID NO;

6 or complements thereof under amplification conditions to generate a first target sequence; and

(b) detecting hybridization between the first target sequence and at least one probe as an indication of the presence of Hepatitis C virus in the test sample, wherein the at least one probe has a sequence selected from the group consisting of;

SEQ ID NO;

7 or SEQ ID NO;

8, or complements thereof.

6. The method of claim 5, wherein the amplification conditions comprise submitting the test sample to an amplification reaction carried out in the presence of suitable amplification reagents.

7. The method of claim 6, wherein the amplification reaction comprises at least one of:

a) PCR;

b) real-time PCR;

or

c) reverse-Transcriptase PCR (RT-PCR).

8. The method of claim 5, wherein, the at least one probe is labeled with a detectable label.

9. The method of claim 8, wherein the detectable label is directly attached to the at least one probe.

10. The method of claim 8, wherein the detectable label is indirectly attached to the at least one probe.

11. The method of claim 8, wherein the detectable label is directly detectable.

12. The method of claim 8, wherein the detectable label is indirectly detectable.

13. The method of claim 8, wherein the detectable label comprises a fluorescent moiety attached at a 5′

end of the at least one probe.

14. The method of claim 13, wherein the at least one probe further comprises a quencher moiety attached at a 3′

end of the at least one probe.

15. The method of claim 5, further comprising the steps of:

(a) contacting the test sample with a forward primer having a sequence of SEQ ID NO;

1 or a complement thereof and a reverse primer having a sequence of SEQ ID NO;

3 or a complement thereof under amplification conditions to generate a second target sequence; and

(b) detecting hybridization between the second target sequence and a probe having a sequence of SEQ ID NO;

7 or a complement thereof as an indication of the presence of HCV in the test sample.

16. A kit for detecting Hepatitis C virus in a test sample, the kit comprising:

(a) at least one forward primer having a sequence selected from the group consisting of;

SEQ ID NO;

1, SEQ ID NO;

2, complements thereof, and any combinations thereof;

(b) at least one reverse primer having a sequence selected from the group consisting of;

SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, complements thereof, and any combinations thereof; and

(c) amplification reagents.

17. The kit of claim 16, further comprising at least one probe, wherein the at least one probe is selected from the group consisting of:

SEQ ID NO;

7, SEQ ID NO;

8, and complements thereof.