Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
6 Assignments
0 Petitions
Accused Products
Abstract
The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.
58 Citations
127 Claims
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1-72. -72. (canceled)
- 73. A cell chip comprising first and second platens, each having a plurality of through-holes, and a porous membrane, wherein the platens are aligned such that the through-holes of the first platen are substantially aligned with the through-holes of the second platen and the membrane is sandwiched in between the two platens.
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76-77. -77. (canceled)
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79-80. -80. (canceled)
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83-84. -84. (canceled)
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88. (canceled)
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90. (canceled)
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92-94. -94. (canceled)
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99. A cell chip comprising in order from top to bottom:
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(a) a coverslip in contact with a spacer; (b) a spacer that separates the covership from a first platen; (c) a first platen having a plurality of through-holes; (d) a gasket comprising a plurality of through-holes that provides a seal between the first platent and the membrane; (e) a porous membrane comprising aluminum oxide and having pores between 0.1 and 1 μ
m sandwiched between the gasket and the second platen;(f) a second platent having a plurality of through holes; (g) a solid support in contact with the second platen. - View Dependent Claims (100, 101, 102)
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107. (canceled)
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113-114. -114. (canceled)
Specification